MISC

2003年7月

ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type I gamma

JOURNAL OF CELL BIOLOGY
  • M Krauss
  • ,
  • M Kinuta
  • ,
  • MR Wenk
  • ,
  • P De Camilli
  • ,
  • K Takei
  • ,
  • Haucke, V

162
1
開始ページ
113
終了ページ
124
記述言語
英語
掲載種別
DOI
10.1083/jcb.200301006
出版者・発行元
ROCKEFELLER UNIV PRESS

Clathrin-mediated endocytosis of synaptic vesicle membranes involves the recruitment of clathrin and AP-2 adaptor complexes to the presynaptic plasma membrane. Phosphoinositides have been implicated in nucleating coat assembly by directly binding to several endocytotic proteins including AP-2 and AP180. Here, we show that the stimulatory effect of ATP and GTPgammaS on clathrin coat recruitment is mediated at least in part by increased levels Of PIP2. We also provide evidence for a role of ADP-ribosylation factor 6 (ARF6) via direct stimulation of a synaptically enriched phosphatidylinositol 4-phosphate 5-kinase type Igamma (PIPKIgamma), in this effect. These data suggest a model according to which activation of PIPKIgamma by ARF6-GTP facilitates clathrin-coated pit assembly at the synapse.


リンク情報
DOI
https://doi.org/10.1083/jcb.200301006
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000184086400009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1083/jcb.200301006
  • ISSN : 0021-9525
  • Web of Science ID : WOS:000184086400009

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