MISC

2004年12月

Immunohistochemical localization of Klotho protein in brain, kidney, and reproductive organs of mice

CELL STRUCTURE AND FUNCTION
  • SA Li
  • ,
  • M Watanabe
  • ,
  • H Yamada
  • ,
  • A Nagai
  • ,
  • M Kinuta
  • ,
  • K Takei

29
4
開始ページ
91
終了ページ
99
記述言語
英語
掲載種別
DOI
10.1247/csf.29.91
出版者・発行元
JAPAN SOC CELL BIOLOGY

Klotho mutant mouse (kl-/-), a mouse model for human aging. exhibits various phenotypes in a wide range of organs including arteriosclerosis, neural degeneration. skin and gonadal atrophy, pulmonary emphysema, calcification of soft tissues, and cognition impairment. Klotho mRNA. however. is expressed only in brain, kidney, reproductive organs, pituitary gland, and parathyroid gland. Therefore it remains to be elucidated how lack of Klotho protein in these limited organs leads to the variery of phenotypes. To shed light on mechanisms by which Klotho protein acts on distant targets, we examined localization of Klotho protein in brain. kidney. and reproductive organs, and analyzed brain and kidney in kl-/- mice searching for changes in target regions in these organs. In brain, Klotho proteins were localized at choroid plexus, where the proteins were dominantly localized at the apical plasma membrane of ependymal cells. In kl-/- brain, reduction of synapses was evident in hippocampus, suggesting a role of Klotho as a humoral factor in cerebrospinal fluid. Klotho proteins in kidney localized at distal renal tubules. Interestingly, in kl-/- mice, type IIa Na/phosphate (Pi) cotransporters, which function at the proximal renal tubules in reabsorption of phosphate ions. were translocated. This suggests that Klotho protein in kidney is implicated in calcium homeostasis which regulates localization of type IIa Na/Pi cotransporters via parathyroid hormone (PTH). Klotho proteins in reproductive organs were expressed only in mature germ cells, although in kl-/- mice germ cell maturation was arrested at earlier stages. Thus, Klotho proteins not only function as a humoral factor, but also are implicated in hormonal regulation. which may explain why mutation of klotho gene results in a variety of phenotypes.


リンク情報
DOI
https://doi.org/10.1247/csf.29.91
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000226532500002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1247/csf.29.91
  • ISSN : 0386-7196
  • eISSN : 1347-3700
  • Web of Science ID : WOS:000226532500002

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