2005年10月
A human beta-cell line for transplantation therapy to control type 1 diabetes
NATURE BIOTECHNOLOGY
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- 巻
- 23
- 号
- 10
- 開始ページ
- 1274
- 終了ページ
- 1282
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1038/nbt1145
- 出版者・発行元
- NATURE PUBLISHING GROUP
A human pancreatic b-cell line that is functionally equivalent to primary b-cells has not been available. We established a reversibly immortalized human b-cell clone ( NAKT-15) by transfection of primary human b-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase ( hTERT) cDNAs flanked by paired loxP recombination targets, which allow deletion of SV40T and TERT by Cre recombinase. Reverted NAKT-15 cells expressed b-cell transcription factors ( Isl-1, Pax 6, Nkx 6.1, Pdx-1), prohormone convertases 1/3 and 2, and secretory granule proteins, and secreted insulin in response to glucose, similar to normal human islets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks; mice remained normoglycemic for longer than 30 weeks. The establishment of this cell line is one step toward a potential cure of diabetes by transplantation.
- リンク情報
- ID情報
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- DOI : 10.1038/nbt1145
- ISSN : 1087-0156
- Web of Science ID : WOS:000232469600029