MISC

2005年10月

A human beta-cell line for transplantation therapy to control type 1 diabetes

NATURE BIOTECHNOLOGY
  • M Narushima
  • ,
  • N Kobayashi
  • ,
  • T Okitsu
  • ,
  • Y Tanaka
  • ,
  • SA Li
  • ,
  • Y Chen
  • ,
  • A Miki
  • ,
  • K Tanaka
  • ,
  • S Nakaji
  • ,
  • K Takei
  • ,
  • AS Gutierrez
  • ,
  • JD Rivas-Carrillo
  • ,
  • N Navarro-Alvarez
  • ,
  • HS Jun
  • ,
  • KA Westerman
  • ,
  • H Noguchi
  • ,
  • JRT Lakey
  • ,
  • P Leboulch
  • ,
  • N Tanaka
  • ,
  • JW Yoon

23
10
開始ページ
1274
終了ページ
1282
記述言語
英語
掲載種別
DOI
10.1038/nbt1145
出版者・発行元
NATURE PUBLISHING GROUP

A human pancreatic b-cell line that is functionally equivalent to primary b-cells has not been available. We established a reversibly immortalized human b-cell clone ( NAKT-15) by transfection of primary human b-cells with a retroviral vector containing simian virus 40 large T-antigen (SV40T) and human telomerase reverse transcriptase ( hTERT) cDNAs flanked by paired loxP recombination targets, which allow deletion of SV40T and TERT by Cre recombinase. Reverted NAKT-15 cells expressed b-cell transcription factors ( Isl-1, Pax 6, Nkx 6.1, Pdx-1), prohormone convertases 1/3 and 2, and secretory granule proteins, and secreted insulin in response to glucose, similar to normal human islets. Transplantation of NAKT-15 cells into streptozotocin-induced diabetic severe combined immunodeficiency mice resulted in perfect control of blood glucose within 2 weeks; mice remained normoglycemic for longer than 30 weeks. The establishment of this cell line is one step toward a potential cure of diabetes by transplantation.


リンク情報
DOI
https://doi.org/10.1038/nbt1145
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000232469600029&DestApp=WOS_CPL
ID情報
  • DOI : 10.1038/nbt1145
  • ISSN : 1087-0156
  • Web of Science ID : WOS:000232469600029

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