2007年11月
Amphiphysin 1 is important for actin polymerization During phagocytosis
MOLECULAR BIOLOGY OF THE CELL
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- 巻
- 18
- 号
- 11
- 開始ページ
- 4669
- 終了ページ
- 4680
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1091/mbc.E07-04-0296
- 出版者・発行元
- AMER SOC CELL BIOLOGY
Amphiphysin 1 is involved in clathrin-mediated endocytosis. In this study, we demonstrate that amphiphysin 1 is essential for cellular phagocytosis and that it is critical for actin polymerization. Phagocytosis in Sertoli cells was induced by stimulating phosphatidylserine receptors. This stimulation led to the formation of actin-rich structures, including ruffles, phagocytic cups, and phagosomes, all of which showed an accumulation of amphiphysin 1. Knocking out amphiphysin 1 by RNA interference in the cells resulted in the reduction of ruffle formation, actin polymerization, and phagocytosis. Phagocytosis was also drastically decreased in amph 1(-/-) Sertoli cells. In addition, phosphatidylinositol-4,5-bisphosphate-induced actin polymerization was decreased in the knockout testis cytosol. The addition of recombinant amphiphysin 1 to the cytosol restored the polymerization process. Ruffle formation in small interfering RNA-treated cells was recovered by the expression of constitutively active Rac1, suggesting that amphiphysin 1 functions upstream of the protein. These findings support that amphiphysin 1 is important in the regulation of actin dynamics and that it is required for phagocytosis.
- リンク情報
- ID情報
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- DOI : 10.1091/mbc.E07-04-0296
- ISSN : 1059-1524
- Web of Science ID : WOS:000250740000040