MISC

2007年10月

cAMP-response element binding protein (CREB) regulates cyclosporine-A-mediated down-regulation of cathepsin B and L synthesis

CELL AND TISSUE RESEARCH
  • Kazuhiro Omori
  • ,
  • Koji Naruishi
  • ,
  • Tomoko Yamaguchi
  • ,
  • Shun-Ai Li
  • ,
  • Mayumi Yamaguchi-Morimoto
  • ,
  • Kaori Matsuura
  • ,
  • Hideo Arai
  • ,
  • Kohji Takei
  • ,
  • Shogo Takashiba

330
1
開始ページ
75
終了ページ
82
記述言語
英語
掲載種別
DOI
10.1007/s00441-007-0457-8
出版者・発行元
SPRINGER

Cyclosporin A (CsA) is an immunosuppressant with severe side effects including gingival overgrowth. We have previously reported that CsA impairs the activity of the lysosomal enzymes cathepsin B and L in human gingival fibroblasts (HGFs). Here, we have examined the effects of CsA on the DNA-binding activity of the cyclic AMP response element-binding protein (CREB) and cell viability, and the effects of CREB on cathepsin B and L synthesis and activity in HGFs. We have confirmed that CsA down-regulates cathepsin B and L synthesis. Further, CsA has no effect on cell viability and dramatically impairs CREB-DNA binding activity. Importantly, the synthesis of cathepsin B and L is down-regulated, and their activity is also significantly impaired in HGFs transfected with plasmid expressing dominant-negative CREB. These results suggest that CREB is essential for the CsA-mediated down-regulation of cathepsin B and L synthesis in HGFs.


リンク情報
DOI
https://doi.org/10.1007/s00441-007-0457-8
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249618700008&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00441-007-0457-8
  • ISSN : 0302-766X
  • Web of Science ID : WOS:000249618700008

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