2002年4月
Utilization of liposomes in vesicular transport studies
CELL STRUCTURE AND FUNCTION
- ,
- 巻
- 27
- 号
- 2
- 開始ページ
- 63
- 終了ページ
- 69
- 記述言語
- 英語
- 掲載種別
- 書評論文,書評,文献紹介等
- DOI
- 10.1247/csf.27.63
- 出版者・発行元
- JAPAN SOC CELL BIOLOGY
Various coated vesicles are implicated in the intracellular transport between different compartments. In vitro reconstitution is a powerful experimental system to study molecular mechanisms involved in assembly of coat proteins from cytosol onto membranes as well as formation of coated vesicles. Liposomes have been recently utilized in the cell-free systems. In this review, we summarize studies on reconstitutions of coated vesicles or coated structures on liposomes. A novel method using dynamic tight scattering (DLS) to quantify vesicle formation from liposomes also is described. Our recent study on the role of phospholipids in vesicle formation, where the DSL assay is used in combination with lipid analysis, also is introduced.
- リンク情報
- ID情報
-
- DOI : 10.1247/csf.27.63
- ISSN : 0386-7196
- eISSN : 1347-3700
- Web of Science ID : WOS:000176510100001