2011年
Dynamin 2 associates with microtubules at mitosis and regulates cell cycle progression.
Cell Structure and Function
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- 巻
- 36
- 号
- 2
- 開始ページ
- 145
- 終了ページ
- 154
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1247/csf.10016
- 出版者・発行元
- Japan Society for Cell Biology
Dynamin, a ~100 kDa large GTPase, is known as a key player for membrane traffic. Recent evidence shows that dynamin also regulates the dynamic instability of microtubules by a mechanism independent of membrane traffic. As microtubules are highly dynamic during mitosis, we investigated whether the regulation of microtubules by dynamin is essential for cell cycle progression. Dynamin 2 intensely localized at the mitotic spindle, and the localization depended on its proline-rich domain (PRD), which is required for microtubule association. The deletion of PRD resulted in the impairment of cytokinesis, whereby the mutant had less effect on endocytosis. Interestingly, dominant-negative dynamin (K44A), which blocks membrane traffic but has no effect on microtubules, also blocked cytokinesis. On the other hand, the deletion of the middle domain, which binds to γ-tubulin, impaired the entry into mitosis. As both deletion mutants had no significant effect on endocytosis, dynamin 2 may participate in cell cycle progression by regulating the microtubules. These data suggest that dynamin may play a key role for cell cycle progression by two distinct pathways, membrane traffic and cytoskeleton.<br>
- リンク情報
- ID情報
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- DOI : 10.1247/csf.10016
- ISSN : 0386-7196
- CiNii Articles ID : 130004137569
- ORCIDのPut Code : 38917389
- ORCIDで取得されたその他外部ID : a:1:{i:0;a:1:{s:0:"";s:0:"";}}