論文

査読有り
2009年12月

Dynamic Interaction of Amphiphysin with N-WASP Regulates Actin Assembly

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Hiroshi Yamada
  • ,
  • Sergi Padilla-Parra
  • ,
  • Sun-Joo Park
  • ,
  • Toshiki Itoh
  • ,
  • Mathilde Chaineau
  • ,
  • Ilaria Monaldi
  • ,
  • Ottavio Cremona
  • ,
  • Fabio Benfenati
  • ,
  • Pietro De Camilli
  • ,
  • Maite Coppey-Moisan
  • ,
  • Marc Tramier
  • ,
  • Thierry Galli
  • ,
  • Kohji Takei

284
49
開始ページ
34244
終了ページ
34256
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.M109.064204
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Amphiphysin 1, an endocytic adaptor concentrated at synapses that couples clathrin-mediated endocytosis to dynamin-dependent fission, was also shown to have a regulatory role in actin dynamics. Here, we report that amphiphysin 1 interacts with N-WASP and stimulates N-WASP- and Arp2/3-dependent actin polymerization. Both the Src homology 3 and the N-BAR domains are required for this stimulation. Acidicliposome-triggered, N-WASP dependent actin polymerization is strongly impaired in brain cytosol of amphiphysin 1 knock-out mice. FRET-FLIM analysis of Sertoli cells, where endogenously expressed amphiphysin 1 co-localizes with N-WASP in peripheral ruffles, confirmed the association between the two proteins in vivo. This association undergoes regulation and is enhanced by stimulating phosphatidylserine receptors on the cell surface with phosphatidylserine-containing liposomes that trigger ruffle formation. These results indicate that actin regulation is a key function of amphiphysin 1 and that such function cooperates with the endocytic adaptor role and membrane shaping/curvature sensing properties of the protein during the endocytic reaction.


リンク情報
DOI
https://doi.org/10.1074/jbc.M109.064204
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000272165200056&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M109.064204
  • ISSN : 0021-9258
  • Web of Science ID : WOS:000272165200056

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