2010年
Use of liposomes to study vesicular transport.
Methods in molecular biology (Clifton, N.J.)
- ,
- ,
- 巻
- 606
- 号
- 開始ページ
- 531
- 終了ページ
- 42
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/978-1-60761-447-0_36
Liposomes have been utilized for variety of membrane transport studies including clathrin-mediated endocytosis. Here we introduce clathrin-coated structures that are generated on large unilamellar liposomes by incubating with clathrin coat proteins. Large unilamellar liposomes are also used to reconstitute vesicle formation in a cell-free system, and the vesicle formation can be quantified by using dynamic light scattering (DLS). Furthermore, phagocytosis assay using liposome-conjugated styrene beads is demonstrated.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-60761-447-0_36
- PubMed ID : 20013420