2006年10月
Interleukin-6 receptor gene polymorphisms and periodontitis in a non-smoking Japanese population
JOURNAL OF CLINICAL PERIODONTOLOGY
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- 巻
- 33
- 号
- 10
- 開始ページ
- 704
- 終了ページ
- 709
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1111/j.1600-051X.2006.00978.x
- 出版者・発行元
- BLACKWELL PUBLISHING
Aim: The indispensable role of interleukin-6 receptor (IL-6R) in regulating IL-6 responses has been clearly established. We have previously reported that IL6R polymorphisms strongly influenced the serum levels of soluble IL-6R. In this study, we investigated the association between these genetic variations and periodontitis.
Material and Methods: Among the seven novel IL6R single-nucleotide polymorphisms (SNPs) reported, we genotyped two important sites: the +48892 A/C in exon 9 and the -183 G/A in the promoter region. The SNP in exon 9 results in Asp -> Ala substitution in the proteolytic cleavage site of IL-6R alpha. In total, 212 periodontitis cases and 210 healthy controls were genotyped using polymerase chain reaction, restriction fragment length polymorphisms and direct sequencing methods.
Results: Analysis of the genotype distribution of the +48892 A/C SNP in periodontitis patients and in controls revealed a suggestive association with aggressive (p=0.04) and chronic periodontitis (p=0.04). In addition, the carriage rate for the A allele was significantly higher in chronic periodontitis patients [p=0.02, odds ratio (OR)=2.25]. No association was found in the -183 G/A SNP. The two markers were in linkage disequilibrium (LD) (|D'|=0.53).
Conclusion: The IL6R +48892 A/C polymorphism could act as a risk factor for periodontitis; however, further association and biological studies are needed.
Material and Methods: Among the seven novel IL6R single-nucleotide polymorphisms (SNPs) reported, we genotyped two important sites: the +48892 A/C in exon 9 and the -183 G/A in the promoter region. The SNP in exon 9 results in Asp -> Ala substitution in the proteolytic cleavage site of IL-6R alpha. In total, 212 periodontitis cases and 210 healthy controls were genotyped using polymerase chain reaction, restriction fragment length polymorphisms and direct sequencing methods.
Results: Analysis of the genotype distribution of the +48892 A/C SNP in periodontitis patients and in controls revealed a suggestive association with aggressive (p=0.04) and chronic periodontitis (p=0.04). In addition, the carriage rate for the A allele was significantly higher in chronic periodontitis patients [p=0.02, odds ratio (OR)=2.25]. No association was found in the -183 G/A SNP. The two markers were in linkage disequilibrium (LD) (|D'|=0.53).
Conclusion: The IL6R +48892 A/C polymorphism could act as a risk factor for periodontitis; however, further association and biological studies are needed.
- リンク情報
- ID情報
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- DOI : 10.1111/j.1600-051X.2006.00978.x
- ISSN : 0303-6979
- PubMed ID : 16899024
- Web of Science ID : WOS:000240288800003