Aug, 2011
Method for Determination of Aflatoxin M-1 in Cheese and Butter by HPLC Using an Immunoaffinity Column
FOOD HYGIENE AND SAFETY SCIENCE
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- Volume
- 52
- Number
- 4
- First page
- 220
- Last page
- 225
- Language
- English
- Publishing type
- Research paper (scientific journal)
- DOI
- 10.3358/shokueishi.52.220
- Publisher
- FOOD HYGIENE & SAFETY
A rapid, sensitive convenient method for determination of aflatoxin M-1 (AFM(1)) in cheese and butter by HPLC was developed and validated. The method employs a safe extraction solution (mixture of acetonitrile, methanol and water) and an immunoaffinity column (IAC) for clean-up. Compared with the widely used method employing chloroform and a Florisil column, the IAC method has a short analytical time and there are no interference peaks. The limits of quantification (LOQ) of the IAC method were 0.12 and 0.14 mu g/kg, while those of the Florisil column method were 0.47 and 0.23 mu g/kg in cheese and buffer, respectively. The recovery and relative standard deviation (RSD) for cheese (spiked at 0.5 mu g/kg) in the IAC method were 92% and 7%, respectively, while for the Florisil column method the corresponding values were 76% and 10%. The recovery and RSD for butter (spiked at 0.5 mu g/kg) in the IAC method were 97% and 9%, and those in the Florisil method were 74% and 9%, respectively. In the IAC method, the values of in-house precision (n=2, day=5) of cheese and butter (spiked at 0.5 mu g/kg) were 9% and 13%, respectively. The IAC method is superior to the Florisil column method in terms of safety, ease of handling, sensitivity and reliability. A survey of AFM(1) contamination in imported cheese and butter in Japan was conducted by the IAC method. AFM(1) not detected in 60 samples of cheese and 30 samples of butter.
- Link information
- ID information
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- DOI : 10.3358/shokueishi.52.220
- ISSN : 0015-6426
- Pubmed ID : 21873812
- Web of Science ID : WOS:000294797600002