Papers

Peer-reviewed
Aug, 2011

Method for Determination of Aflatoxin M-1 in Cheese and Butter by HPLC Using an Immunoaffinity Column

FOOD HYGIENE AND SAFETY SCIENCE
  • Hisako Sakuma
  • ,
  • Yoichi Kamata
  • ,
  • Yoshiko Sugita-Konishi
  • ,
  • Hiroshi Kawakami

Volume
52
Number
4
First page
220
Last page
225
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.3358/shokueishi.52.220
Publisher
FOOD HYGIENE & SAFETY

A rapid, sensitive convenient method for determination of aflatoxin M-1 (AFM(1)) in cheese and butter by HPLC was developed and validated. The method employs a safe extraction solution (mixture of acetonitrile, methanol and water) and an immunoaffinity column (IAC) for clean-up. Compared with the widely used method employing chloroform and a Florisil column, the IAC method has a short analytical time and there are no interference peaks. The limits of quantification (LOQ) of the IAC method were 0.12 and 0.14 mu g/kg, while those of the Florisil column method were 0.47 and 0.23 mu g/kg in cheese and buffer, respectively. The recovery and relative standard deviation (RSD) for cheese (spiked at 0.5 mu g/kg) in the IAC method were 92% and 7%, respectively, while for the Florisil column method the corresponding values were 76% and 10%. The recovery and RSD for butter (spiked at 0.5 mu g/kg) in the IAC method were 97% and 9%, and those in the Florisil method were 74% and 9%, respectively. In the IAC method, the values of in-house precision (n=2, day=5) of cheese and butter (spiked at 0.5 mu g/kg) were 9% and 13%, respectively. The IAC method is superior to the Florisil column method in terms of safety, ease of handling, sensitivity and reliability. A survey of AFM(1) contamination in imported cheese and butter in Japan was conducted by the IAC method. AFM(1) not detected in 60 samples of cheese and 30 samples of butter.

Link information
DOI
https://doi.org/10.3358/shokueishi.52.220
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21873812
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000294797600002&DestApp=WOS_CPL
ID information
  • DOI : 10.3358/shokueishi.52.220
  • ISSN : 0015-6426
  • Pubmed ID : 21873812
  • Web of Science ID : WOS:000294797600002

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