2017年9月19日
RNA localization is a key determinant of neurite-enriched proteome.
Nature communications
- 巻
- 8
- 号
- 1
- 開始ページ
- 583
- 終了ページ
- 583
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/s41467-017-00690-6
- 出版者・発行元
- NATURE PUBLISHING GROUP
Protein subcellular localization is fundamental to the establishment of the body axis, cell migration, synaptic plasticity, and a vast range of other biological processes. Protein localization occurs through three mechanisms: protein transport, mRNA localization, and local translation. However, the relative contribution of each process to neuronal polarity remains unknown. Using neurons differentiated from mouse embryonic stem cells, we analyze protein and RNA expression and translation rates in isolated cell bodies and neurites genome-wide. We quantify 7323 proteins and the entire transcriptome, and identify hundreds of neurite-localized proteins and locally translated mRNAs. Our results demonstrate that mRNA localization is the primary mechanism for protein localization in neurites that may account for half of the neurite-localized proteome. Moreover, we identify multiple neurite-targeted non-coding RNAs and RNA-binding proteins with potential regulatory roles. These results provide further insight into the mechanisms underlying the establishment of neuronal polarity.Subcellular localization of RNAs and proteins is important for polarized cells such as neurons. Here the authors differentiate mouse embryonic stem cells into neurons, and analyze the local transcriptome, proteome, and translated transcriptome in their cell bodies and neurites, providing a unique resource for future studies on neuronal polarity.
- リンク情報
-
- DOI
- https://doi.org/10.1038/s41467-017-00690-6
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/28928394
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5605627
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000411166800002&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1038/s41467-017-00690-6
- ISSN : 2041-1723
- PubMed ID : 28928394
- PubMed Central 記事ID : PMC5605627
- Web of Science ID : WOS:000411166800002