Kouhei Nagai

J-GLOBAL         Last updated: Oct 11, 2019 at 16:39
 
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Name
Kouhei Nagai
Affiliation
Kindai University
Section
Faculty of Biology-Oriented Science and Technology Department of Genetic Engineering
Job title
Associated professor
Degree
Ph.D(Kyoto University)
Research funding number
70500578

Research Areas

 
 

Academic & Professional Experience

 
Apr 2016
 - 
Today
Associated professor, BOST, Kindai University
 
Jul 2012
 - 
Mar 2016
Wakayama Industry promotion fundation
 
Apr 2012
 - 
Mar 2015
Lecturer, BOST, Kindai University
 
Sep 2009
 - 
Mar 2012
Assistant professor, St. Mariannna University of Medicine
 
Apr 2005
 - 
Aug 2009
Post Doctoral Fellow, Wakayama Industry promotion fundation
 

Education

 
Apr 2001
 - 
Mar 2004
Fuculty of Agriculture, Graduate School of Kyoto University
 
Apr 1999
 - 
Mar 2001
Fuculty of Agriculture, Graduate School of Kyoto University
 
Apr 1996
 - 
Mar 1999
Fuculty of Agriculture, Kyoto University
 

Published Papers

 
Kazuo Yamagata, Kouhei Nagai, Hiroshi Miyamoto, Masayuki Anzai, Hiromi Kato, Kei Miyamoto, Satoshi Kurosaka, Rika Azuma, Igor I. Kolodeznikov, Albert V. Protopopov, Valerii V. Plotnikov, Hisato Kobayashi, Ryouka Kawahara-Miki, Tomohiro Kono, Masao Uchida, Yasuyuki Shibata, Tetsuya Handa, Hiroshi Kimura, Yoshihiko Hosoi, Tasuku Mitani, Kazuya Matsumoto & Akira Iritani
Scientific Reports   9 4050   Mar 2019   [Refereed]
Morita K, Tokoro M, Hatanaka Y, Higuchi C, Ikegami H, Nagai K, Anzai M, Kato H, Mitani T, Taguchi Y, Yamagata K, Hosoi Y, Miyamoto K, Matsumoto K.
J Reprod Dev.   64(2) 161-171   Apr 2018   [Refereed]
Higuchi C, Shimizu N, Shin SW, Morita K, Nagai K, Anzai M, Kato H, Mitani T, Yamagata K, Hosoi Y, Miyamoto K, Matsumoto K.
J Reprod Dev.   64(1) 65-74   Dec 2017   [Refereed]
Matsuura T, Sato M, Nagai K, Sato T, Arito M, Omoteyama K, Naoya Suematsu, Okamoto K, Kato T, Soma Y, Kurokawa MS.
Journal of Dermatological Science   87 36-49   2017   [Refereed]
Altered acetylation of proteins in patients with rheumatoid arthritis, revealed by acetyl-proteomics
Arito M, Nagai K, Ooka S, Sato T, Takakuwa Y, Kurokawa MS, Okamoto K, Suematsu N, Kato T
Arthritis Research & Therapy   33(6) 877-886   2015   [Refereed]
Ikegami H, Nagai K, Matsuhashi T, Kobayash N, Takemoto A, Yoshihiro T, Inoue E, Higuchi T, Morita K, Uchibori S, Amano T, Y Taguchi, Kato H, A Iritani, Matsumoto K
Japanese Society of Animal Science   86 141-152   2015   [Refereed]
Protein profiles of peripheral blood mononuclear cells as a candidate biomarker for Bechet’s disease.
Yoshioka T, Kurokawa MS, Sato T, Nagai K, Iizuka N, Arito M, Takakuwa Y, Nakano H, Ooka S, Suematsu N, Okamoto K, Yudph K, Nakamura H, Suzuki N, Ozaki S, Kato T
Clin Exp. Rheumatol.   32 S9-S19   2014   [Refereed]
Noguchi M, Sato T, Nagai K, Utagawa I, Suzuki I, Arito M, Iizuka N, Suematsu N, Okamoto K, Kato T, Yamaguchi N, Kurokawa MS
Int. J. Geriatr Psychiatry   29 808-818   2014   [Refereed]
Uchida T, Nagai K, Sato T, Iizuka N, Arito M, Takakuwa Y, Nakano H, Ooka S, Kurokawa MS, Suematsu N, Okamoto K, Ozaki S, Kato T
J. Proteomics   91 259-269   2013   [Refereed]

Misc

 
Ume polyphenol: prebiotic effects on diet-induced obesity mice and growth-promoting mechanism for bifidobacteria.
Masahiro Kumeno, Azusa Ito, Natsumi Ohigashi, Yuki Yoshihara, Toshio Suzuki, Kouhei Nagai, Hisashi Ashida
Mem. Faculity. B.O.S.T. Kindai University   42 1-14   Nov 2018   [Refereed]
Comprehensive quantification of liver proteins in the high-fat induced-obese mouse using a SWATH acquisition method
Shiomi Oota, Tomoya Nishibata, Sayaka Yamawaki, Tomoki Awaji, Yuki Oosedo, Ayaka Sakaue, Kunihiro Kishida, Kouhei Nagai
Mem. Faculity. B.O.S.T. Kindai University   42 15-32   Nov 2018   [Refereed]
Study on preservation of animal tissue provided from zoos : Toward knowledge sharing with the zoos and aquariums
Masayuki Anzai, Rika Azuma, Moriyoshi Kubo, Noriyuki Nonoue, Kazutoshi Takami, Minoru Miyashita, Hitoshi Murai, Kouhei Nagai, Akari Obashi, Takuya Orisugi, Yoshihiko Hosoi
Memoirs of Institute of Advanced Technology, Kindai University   22 25-34   Mar 2017   [Refereed]
なし
Kouhei Nagai
Inflammation&Immunity   24(2) 95-99   Mar 2016   [Invited]
NAGAI KOHEI, SAITO KAZUKI, ARITO MITSUMI, KUROKAWA MANAE, KATO TOMOHIRO
Mem Fac Biol Oriented Sci Technol Kinki Univ   (35) 7-16   Mar 2015
MITSUI HIROYUKI, OKAMOTO KAZUKI, KUROKAWA MANAE, ARITO MITSUMI, NAGAI KOHEI, SATO TOSHIYUKI, IIZUKA MICHIKO, YUDO KAZUO, BEPPU MOROE, KATO TOMOHIRO
日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集   56th-21st 325   Mar 2012
SATO TOSHIYUKI, TAKAKUWA YUKIKO, OOKA SEIDO, NAGAI KOHEI, ARITO MITSUMI, IIZUKA MICHIKO, KUROKAWA MANAE, OKAMOTO KAZUKI, KATO TOMOHIRO
日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集   56th-21st 486   Mar 2012
KUROKAWA MANAE, TAKENOUCHI KENJI, NAKAMURA YO, OKAMOTO KAZUKI, ARITO MITSUMI, SATO TOSHIYUKI, NAGAI KOHEI, YUDO KAZUO, KATO TOMOHIRO
日本リウマチ学会総会・学術集会・国際リウマチシンポジウムプログラム・抄録集   56th-21st 343   Mar 2012
SHIMIZU NATSUMI, HATANAKA YUKI, HIGUCHI CHIKA, SHIN SEUNG-WOOK, NISHIKAWA SATOSHI, NISHIHARA TAKUJI, KATO RIE, TAKEMOTO ATSUSHI, MORITA KOTARO, NAGAI KOHEI, AMANO TOMOKO, KISHIGAMI SATOSHI, ANZAI MASAYUKI, HOSOI YOSHIHIKO, MATSUMOTO KAZUYA
日本分子生物学会年会プログラム・要旨集(Web)   35th 3P-0488 (WEB ONLY)   2012

Conference Activities & Talks

 
Comprehensive analysis of the effects of high-fat diet on protein abundance in the mouse liver using SWATH acquisition method.
S. Yamawaki, N. Misawa, T. Nishibata, R. Nagamine, T. Awaji, Y. Oosedo, A. Sakaue, K. Kishida, K. Nagai
JPROS2019   26 Jul 2019   
Comprehensive analysis of post-translational modifications on myeloperoxidase from patients with ANCA associated vasculitis.
R. Sasaki, K. Nagai, T. Nishibata, M. Sato, T. Sato, M. S. Kurokawa, T. Kato
JPROS2019   26 Jul 2019   
Comprehensive analysis of post-translational modifications on the proteins from a 28,000-year-old woolly mammoth
T. Nishibata, K. Nagai, K. Yamagata, H. Miyamoto, M. Anzai, H. Kato, K. Miyamoto, R. Azuma, I. I. Kolodeznikov, A. V. Protopopov, V. V. Plotnikov, Y. Hosoi, T. Mitani, K. Matsumoto, A. Iritani
JPROS2019   26 Jul 2019   
Proteomic analysis of muscle and bone marrow tissues obtained from a 28,000-year-old woolly mammoth.
K. Nagai, H. Miyamoto, M. Anzai, R. Azuma, T. Nishibata, K. Yamagata, H. Kato, K. Miyamoto, I. I. Kolodeznikov, A. V. Protopopov, V. V. Plotnikov, Y. Hosoi, T. Mitani, K. Matsumoto, A. Iritani.
JPROS2019   25 Jul 2019   
Comprehensive quantification of liver proteins in the high-fat induced-obese mouse using a SWATH-MS analysis [Invited]
Tomoya Nishibata, Sayaka Yamawaki, Siomi Oota, Tomoki Awaji, Yuki Oosedo, Ayaka Sakaue, Kunihiro Kishida, Kouhei Nagai
25 Sep 2018   
Discovery of biomarker candidate proteins for predicting beef carcass traits and meat quality characteristics by time-course proteomic analysis of serum in Japanese Black steers using SWATH-MS and Lasso regression analysis.
H. Ikegami, T. Matsuhashi, K. Ochi, H. Sano, K. Nagai, K. Obayashi, H. Kato, S. Sakaguchi, T. Yoshihiro, K. Masumoto.
MSP2018   16 May 2018   
Assessment of reproducibility and quantitative performance of SWATH-mass spectrometry of bovine serum proteins.
K. Makino, T. Nishibata, S. Yamawaki, H. Ikegami, T. Matsuhashi, K. Nagai, K. Matsumoto
MSP2018   16 May 2018   
Comprehensive quantification of liver proteins in the high-fat induced-obese mouse using a SWATH acquisition method – Toward elucidation of molecular mechanism of functional food substances-
S. Oota, ○S. Yamawaki, T. Nishibata, T. Awaji, Y. Oosedo, A. Sakaue, K. Kishida, K. Nagai
MSP2018   15 May 2018   
New extraction approach of non-collagenous proteins from bone tissue by mild-degradation of enzyme
Saori Kunii, Kouhei Nagai, Koichi Morimoto
ECTS 2017   14 May 2017   
Oxidative modification of myeloperoxidase in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitides
Manae S. Kurokawa, Kouhei Nagai, Toshiyuki Sato, Masaaki Sato, Yukiko Takakuwa, Seido Ooka, Mitsumi Arito, Tomohiro Kato
The 18th International Vasculitis and ANCA Workshop   Mar 2017   
Prediction of beef carcass traits using changes in amount of serum proteins during fattening period
H ikegami, T Matsuhashi, K Nagai, M Morimoto, T Tsukamoto, S Yamaguchi, C Higuchi, K Morita, S Sakamoto, K Matsumoto,
JPROS2016   29 Jul 2016   
なし
Kouhei Nagai
21 Apr 2016   
なし
Kouhei Nagai, Nobuhei Ryu, Koji Yoshida, Tsuyoshi Itani, Mizuki Higashiyama
29 Mar 2016   
第37回日本分子生物学会
Aug 2014   
Comparative proteomic analysis of neutrophils from patients with microscopic polyangiitis and granulomatosis with polyangiitis.
Teisuke Uchida, Kouhei Nagai, Toshiyuki Sato, Nobuko Iizuka, Mitsumi Arito, Yukiko Takakuwa, Hirosama Nakano, Seido Ooka, Manae Kurokawa, Naoya Suematsu, Kazuki Okamoto, Shoichi Ozaki, Tomohiro Kato
ACR/ARHP annual meeting 2013   Oct 2013   
Protein profilrs of peripheral blood mononuclear cells as a biomarker for bechet's disease.
Takuya Yoshioka, Manae S. Kurokawa, Toshiyuki Sato, Kouhei Nagai, Nobuko Iizuka, Mitsumi Arito, Yukiko Takakuwa, Hiromasa Nakano, Seido Ooka, Naoya Suematsu, Kazuki Okamoto
ACR/ARHP annual meeting 2013   Oct 2013   
Serum Peptides, Represented by Complement 3f Des-Arginine, Are Useful for Prediction of the Response to Pegylated Interferon-α Plus Ribavirin in Patients with Chronic Hepatitis C
Yohei Noguchi, Manae S. Kurokawa, Chiaki Okuse, Nobuyuki Matsumoto, Kouhei Nagai, Toshiyuki Sato, Mitsumi Arito, Naoya Suematsu, Kazuki Okamoto, Michihiro Suzuki, Fumio Itoh, Tomohiro Kato
HUPO2013   Sep 2013   
Comparative Proteomic Analysis of Neutrophils from Patients with Microscopic Polyangiitis and Granulomatosis with Polyangiitis
Teisuke Uchida, Kouhei Nagai, Toshiyuki Sato, Nobuko Iizuka, Mitsumi Arito, Yukiko Takakuwa, Hirosama Nakano, Seido Ooka, Manae Kurokawa, Naoya Suematsu, Kazuki Okamoto, Shoichi Ozaki, Tomohiro Kato
Sep 2013   
Biomarker Discovery from Low Abundance Proteins in Bovine Serum; Proteomics for Developing Beef Production Systems
Haruka Ikegami, Kouhei Nagai, Atsushi Takemoto, Natsumi Shimizu, Kohtaro Morita, Chika Higuchi, Tamako Matsuhashi, Naohiko Kobayashi, Kazuya Matsumoto
Sep 2013   
Identification of Proteins Related to Accumulation of Intramuscular Fat in Japanese Black by Proteomic Analysis
Atsushi Takemoto, Kouhei Nagai, Haruka Ikegami, Natsumi Shimizu, Kohtaro Morita, Chika Higuchi, Eiji Kobayashi, Kazuya Matsumoto
Sep 2013   
Protein Profiles of Peripheral Blood Mononuclear Cells as a Biomarker for Behcet’s Disease
Takuya Yoshioka, Manae S. Kurokawa, Toshiyuki Sato, Kouhei Nagai, Nobuko Iizuka, Mitsumi Arito, Yukiko Takakuwa, Hiromasa Nakano, Seido Ooka, Naoya Suematsu, Kazuki Okamoto
HUPO2013   Sep 2013   
Comprehensive Analysis of Aberrantly Glycosylated Proteins in Rheumatoid Arthritis
Toshiyuki Sato, Mitsumi Arito, Manae S. Kurokawa, Yukiko Takakuwa, Seido Ooka, Kouhei Nagai, Hiroshi Nakamura, Nobuko Iizuka, Naoya Suematsu, Kazuki Okamoto, Tomohiro Kato
HUPO2013   Sep 2013   
Proteomic analysis of bovine serum: Discovery of biomarker proteins evaluating carcass and meat quality traits in Japanese Black beef cattle.
Kouhei NAGAI, Haruka IKEGAMI, Atsushi TAKEMOTO, Natsumi SHIMIZU, Kohtaro MORITA, Chika HIGUCHI, Eiji KOBAYASHI and Kazuya MATSUMOTO
Animal Science Day 2013   Sep 2013   
Proteomic analysis of muscle tissue for discovery of proteins related to accumulation of intramuscular fat in beef cattle
Atsushi TAKEMOTO, Kouhei NAGAI, Haruka IKEGAMI, Natsumi SHIMIZU, Kohtaro MORITA, Chika HIGUCHI, Eiji KOBAYASHI and Kazuya MATSUMOTO
Animal Science Day 2013   Sep 2013   
Altered acetylation of proteins in patients with rheumatoid arthritis, revealed by acetyl-proteomics.
Arito M, Nagai K, Ooka S, Sato T, Takakuwa Y, Kurokawa MS, Okamoto Suematsu N, Arito M, Nagai K, Ooka S, Sato T, Takakuwa Y, Kurokawa MS, Okamoto Suematsu N, Kato T.
Dec 2012   
Comprehensive analysis of protein expression in peripheral blood mononuclear cells from patients with Bechet's Disease.
Kouhei NagaiYoshioka T, Kurokawa MS, Sato T, Iiduka N, Arito M, Nagai K, Suematsu N, Okamoto K, Kato T.
ACR/ARHP annual meeting 2012   Oct 2012   
Comparative proteomic analysis of neutrophils between microscopic polyangiitis and granulomatosis with polyandiiti
Yoshioka T, Kurokawa MS, Sato T, Iiduka N, Arito M, Nagai K, Suematsu N, Okamoto K, Kato T.
ACR/ARHP annual meeting 2012   Oct 2012   
Comprehensive analysis of serum peptides in patients with Alzheimer's Disease.
Noguchi M, Kurokawa MS, Utagawa I, Nagai K, Sato T, Arito M, Suematsu N, Okamoto K, Yamaguchi N, Kato T
Sep 2012   
Oxidative modification on myeloperoxidase in patients with Anineutrophil cytoplasmic antibody (ANCA)-associated Vascullitides.
Nagai K, Uchida T, Takakuwa Y, Ooka S, Arito M, Satoh T, Kurokawa MS, Okamoto K, Suematsu N, Kato T.
HUPO2012   Sep 2012   
Adenosine Deaminase identified as a citrullination-dependent autoantigen in rheumatoid arthritis, by proteomic surveillance with in vitro citrullination.
Kato T, Mitsui H, Arito M, Sato T, Suematsu N, Okamoto K, Kurokawa MS, Yudo K, Nagai K, Beppu M
HUPO2012   Sep 2012   
Comprehensive analysis of protein expression in peripheral blood mononuclear cells from patients with Bechet's disease.
Kurokawa MS, Yoshioka T, Sato T, Iiduka N, Arito M, Nagai K, Suematsu N, Okamoto K, Kato T
HUPO2012   Sep 2012   
Comparative proteomic analysis of neutrophils from patients with microscopic polyangiitis and granulomatosis with polyangiitis.
Uchida T, Nagai K, Takakuwa Y, Ooka S, Arito M, Satoh T, Kurokawa MS, Okamoto K, Suematsu N, Kato T.
HUPO2012   Sep 2012   
Screening of glycoproteins with altered glycans in rheumatoid arthritis.
Sato T, Ooka S, Takakuwa Y, Nagai K, Arito M, Iizuka N, Kurokawa MS, Okamoto K, Suematsu N, Kato T.
HUPO2012   Sep 2012   
Altered acetylation of proteins in patients with rheumatoid arthritis, revealed by acetyl-proteomics.
Arito M, Nagai K, Ooka S, Takakuwa Y, Kurokawa MS, Sato T, Iiduka N, Okamoto K, Suematsu N, Kato T.
HUPO2012   Sep 2012   
日本プロテオーム学会 日 2012年大会
Jul 2012   
Comparative proteomic analysis of neutrophils from patients with microscopic polyangiitis and granulomatosis with polyangiitis.
Teisuke Uchida,Kouhei Nagai,Yukiko Takakuwa ,
Apr 2012   

Research Grants & Projects

 
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(若手研究(B))
Project Year: 2012 - 2013    Investigator(s): Mitsumi ARITO
Post-translational modifications (PTMs) are often critical for diagnosis of diseases. Thereby, we here tried to elucidate alteration of PTMs in RA, focusing on acetylation in this study.We applied acetyl-proteomics to peripheral blood mononuclear ...
Ministry of Education, Culture, Sports, Science and Technology: Grants-in-Aid for Scientific Research(若手研究(B))
Project Year: 2012 - 2013    Investigator(s): Kouhei NAGAI
In this study, we tried to establish an useful method for detecting a de-phosphorilated form of U1-68k autoimmunogen, which was found to be increased in peripheral blood mononuclear cells of patients of systemic lupus erythematosus or Mixed connec...