論文

査読有り 国際誌
2022年10月7日

Proton gradient mediates hemolymph trehalose influx into aphid bacteriocytes

Archives of Insect Biochemistry and Physiology
  • Mana Iwata
  • ,
  • Mayu Yoshinaga
  • ,
  • Kosuke Mizutani
  • ,
  • Takahiro Kikawada
  • ,
  • Shingo Kikuta

開始ページ
e21971
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/arch.21971
出版者・発行元
Wiley

Aphids harbor proteobacterial endosymbionts such as Buchnera aphidicola housed in specialized bacteriocytes derived from host cells. The endosymbiont Buchnera supplies essential amino acids such as arginine to the host cells and, in turn, obtains sugars needed for its survival from the hemolymph. The mechanism of sugar supply in aphid bacteriocytes has been rarely studied. It also remains unclear how Buchnera acquires its carbon source. The hemolymph sugars in Acyrthosiphon pisum are composed of the disaccharide trehalose containing two glucose molecules. Here, we report for the first time that trehalose is transported and used as a potential carbon source by Buchnera across the bacteriocyte plasma membrane via trehalose transporters. The current study characterized the bacteriocyte trehalose transporter Ap_ST11 (LOC100159441) using the Xenopus oocyte expression system. The Ap_ST11 transporter was found to be proton-dependent with a Km value ≥700 mM. We re-examined the hemolymph trehalose at 217.8 mM using a fluorescent trehalose sensor. The bacteriocytes did not obtain trehalose by facilitated diffusion along the gradient across cellular membranes. These findings suggest that trehalose influx into the bacteriocytes depends on the extracellular proton-driven secondary electrochemical transporter.

リンク情報
DOI
https://doi.org/10.1002/arch.21971
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36205078
URL
https://onlinelibrary.wiley.com/doi/pdf/10.1002/arch.21971
URL
https://onlinelibrary.wiley.com/doi/full-xml/10.1002/arch.21971
ID情報
  • DOI : 10.1002/arch.21971
  • ISSN : 0739-4462
  • eISSN : 1520-6327
  • PubMed ID : 36205078

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