2008年3月
Purification and characterization of a rice class I chitinase, OsChia1b, produced in Esherichia coli
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 72
- 号
- 3
- 開始ページ
- 893
- 終了ページ
- 895
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1271/bbb.706931
- 出版者・発行元
- TAYLOR & FRANCIS LTD
To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica sp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.706931
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 18323646
- Web of Science ID : WOS:000254693300036