2010年5月
Improved Transformation of the Halo-Tolerant Yeast Zygosaccharomyces rouxii by Electroporation
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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- 巻
- 74
- 号
- 5
- 開始ページ
- 1092
- 終了ページ
- 1094
- 記述言語
- 英語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1271/bbb.90865
- 出版者・発行元
- TAYLOR & FRANCIS LTD
To improve the transformation efficiency of Zygosaccharomyces rouxii by electroporation, glycerol was added to the electroporation buffer and the cells were frozen at -80 degrees C. These alterations drastically increased transformation efficiency, and we found that competent cells can be preserved at -80 degrees C without decreasing their transformation efficiency for at least 30d.
- リンク情報
- ID情報
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- DOI : 10.1271/bbb.90865
- ISSN : 0916-8451
- eISSN : 1347-6947
- PubMed ID : 20460697
- Web of Science ID : WOS:000278356700032