論文

査読有り 国際誌
2017年1月

Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies.

Anticancer research
  • Kazuhiko Shien

37
1
開始ページ
301
終了ページ
307
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.21873/anticanres.11321
出版者・発行元
INT INST ANTICANCER RESEARCH

BACKGROUND: Reduced expression in immortalized cell (REIC)/Dickkoph-3 (DKK3) is a tumor-suppressor gene, and its overexpression by adenovirus vector (Ad-REIC) exhibits a remarkable therapeutic effect on various human cancer types through a mechanism triggered by endoplasmic reticulum stress. MATERIALS AND METHODS: We examined the direct anti-tumor effect of Ad-REIC gene therapy on lung cancer and malignant mesothelioma cell lines in vitro, and the distant bystander effect using immunocompetent mouse allograft models with bilateral flank tumors. RESULTS: Ad-REIC treatment showed antitumor effect in many lung cancer and malignant mesothelioma cell lines in vitro. In an in vivo model, Ad-REIC treatment inhibited the growth not only of directly treated tumors but also of distant untreated tumors. By immunohistochemical analysis, infiltration of T-cells and natural killer (NK) cells and expression of the major histocompatibility complex (MHC) class I molecules were observed in bilateral tumors. CONCLUSION: Ad-REIC treatment not only had a direct antitumor effect but also an indirect bystander effect through stimulation of the immune system.

リンク情報
DOI
https://doi.org/10.21873/anticanres.11321
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/28011506
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000391958800040&DestApp=WOS_CPL
URL
http://europepmc.org/abstract/med/28011506
ID情報
  • DOI : 10.21873/anticanres.11321
  • ISSN : 0250-7005
  • eISSN : 1791-7530
  • ORCIDのPut Code : 38241025
  • PubMed ID : 28011506
  • Web of Science ID : WOS:000391958800040

エクスポート
BibTeX RIS