2016年9月
Detection of Sphingomyelin Clusters by Raman Spectroscopy
BIOPHYSICAL JOURNAL
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 111
- 号
- 5
- 開始ページ
- 999
- 終了ページ
- 1007
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bpj.2016.07.035
- 出版者・発行元
- CELL PRESS
Sphingomyelin (SM) is a major sphingolipid in mammalian cells that forms specific lipid domains in combination with cholesterol (Chol). Using molecular-dynamics simulation and density functional theory calculation, we identified a characteristic Raman band of SM at similar to 1643 cm(-1) as amide I of the SM cluster. Experimental results indicate that this band is sensitive to the hydration of SM and the presence of Chol. We showed that this amide I Raman band can be utilized to examine the membrane distribution of SM. Similarly to SM, ceramide phosphoethanolamine (CerPE) exhibited an amide I Raman band in almost the same region, although CerPE lacks three methyl groups in the phosphocholine moiety of SM. In contrast to SM, the amide I band of CerPE was not affected by Chol, suggesting the importance of the methyl groups of SM in the SM-Chol interaction.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.bpj.2016.07.035
- ISSN : 0006-3495
- eISSN : 1542-0086
- Web of Science ID : WOS:000382801300015