論文

国際誌
2021年12月20日

A widely distributed HIV-1 provirus elimination assay to evaluate latency-reversing agents in vitro.

Cell reports methods
  • Kouki Matsuda
  • Saiful Islam
  • Toru Takada
  • Kiyoto Tsuchiya
  • Benjy Jek Yang Tan
  • Shin-Ichiro Hattori
  • Hiroo Katsuya
  • Kosaku Kitagawa
  • Kwang Su Kim
  • Misaki Matsuo
  • Kenji Sugata
  • Nicole S Delino
  • Hiroyuki Gatanaga
  • Kazuhisa Yoshimura
  • Shuzo Matsushita
  • Hiroaki Mitsuya
  • Shingo Iwami
  • Yorifumi Satou
  • Kenji Maeda
  • 全て表示

1
8
開始ページ
100122
終了ページ
100122
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.crmeth.2021.100122

Persistence of HIV-1 latent reservoir cells during antiretroviral therapy (ART) is a major obstacle for curing HIV-1. Even though latency-reversing agents (LRAs) are under development to reactivate and eradicate latently infected cells, there are few useful models for evaluating LRA activity in vitro. Here, we establish a long-term cell culture system called the "widely distributed intact provirus elimination" (WIPE) assay. It harbors thousands of different HIV-1-infected cell clones with a wide distribution of HIV-1 provirus similar to that observed in vivo. Mathematical modeling and experimental results from this in vitro infection model demonstrates that the addition of an LRA to ART shows a latency-reversing effect and contributes to the eradication of replication-competent HIV-1. The WIPE assay can be used to optimize therapeutics against HIV-1 latency and investigate mechanistic insights into the clonal selection of heterogeneous HIV-1-infected cells.

リンク情報
DOI
https://doi.org/10.1016/j.crmeth.2021.100122
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/35475215
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9017183
ID情報
  • DOI : 10.1016/j.crmeth.2021.100122
  • PubMed ID : 35475215
  • PubMed Central 記事ID : PMC9017183

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