2008年
Loss-of-function analyses of the fragile X-related and dopamine receptor genes by RNA interference in the cricket Gryllus bimaculatus
Developmental Dynamics
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- 巻
- 238
- 号
- 8
- 開始ページ
- 2025
- 終了ページ
- 2033
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/dvdy.22029
- 出版者・発行元
- WILEY-BLACKWELL
In order to explore a possibility that the cricket Gryllus bimaculatus would be a useful model to unveil molecular mechanisms of human diseases, we performed loss-of-function analyses of Gryllus genes homologous to human genes that are responsible for human disorders, fragile X mental retardation 1 (fmr1) and Dopamine receptor (DopR). We cloned cDNAs of their Gryllus homologues, Gb'fmr1, Gb'DopRI, and Gb'DopRII, and analyzed their functions with use of nymphal RNA interference (RNAi). For Gb'fmr1, three major phenotypes were observed: (1) abnormal wing postures, (2) abnormal calling song, and (3) loss of the circadian locomotor rhythm, while for Gb'DopRI, defects of wing posture and morphology were found. These results indicate that the cricket has the potential to become a novel model system to explore human neuronal pathogenic mechanisms and to screen therapeutic drugs by RNAi. Developmental Dynamics 238. 2025-2033, 2009. (C) 2009 Wiley-Liss, Inc.
- リンク情報
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- DOI
- https://doi.org/10.1002/dvdy.22029
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000268685100014&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-67650796184&partnerID=MN8TOARS
- ID情報
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- DOI : 10.1002/dvdy.22029
- ISSN : 1058-8388
- ORCIDのPut Code : 28249600
- SCOPUS ID : 67650796184
- Web of Science ID : WOS:000268685100014