2009年3月
Identification and functional analysis of pistil self-incompatibility factor HT-B of Petunia
JOURNAL OF EXPERIMENTAL BOTANY
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- 巻
- 60
- 号
- 4
- 開始ページ
- 1309
- 終了ページ
- 1318
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jxb/erp005
- 出版者・発行元
- OXFORD UNIV PRESS
Gametophytic self-incompatibility (GSI) in Solanaceae, Rosaceae, and Plantaginaceae is controlled by a multiallelic S-locus. The specificities of pistil and pollen are controlled by separate S-locus genes, S-RNase and SLF/SFB, respectively. Although the S-specificity is determined by the S-locus genes, factors located outside the S-locus are also required for expression of GSI. HT-B is one of the pistil non-S-factors identified in Nicotiana and Solanum, and encodes a small asparagine/aspartate-rich extracellular protein with unknown biochemical function. Here, HT-B was cloned from Petunia and characterized. The structural features and expression pattern of Petunia HT-B were very similar to those of Nicotiana and Solanum. Unlike other solanaceous species, expression of HT-B was also observed in self-compatible Petunia species. RNA interference (RNAi)-mediated suppression of Petunia HT-B resulted in partial breakdown of GSI. Quantitative analysis of the HT-B mRNA accumulation in the transgenics showed that a 100-fold reduction is not sufficient and a > 1000-fold reduction is required to achieve partial breakdown of GSI.
- リンク情報
- ID情報
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- DOI : 10.1093/jxb/erp005
- ISSN : 0022-0957
- Web of Science ID : WOS:000264390600024