2017年
Structural Analysis of Long Single-Stranded RNA Molecules with Atomic Force Microscopy Imaging
3RD INTERNATIONAL MULTIDISCIPLINARY MICROSCOPY AND MICROANALYSIS CONGRESS (INTERM)
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- 巻
- 186
- 号
- 開始ページ
- 3
- 終了ページ
- 9
- 記述言語
- 英語
- 掲載種別
- 研究論文(国際会議プロシーディングス)
- DOI
- 10.1007/978-3-319-46601-9_1
- 出版者・発行元
- SPRINGER INT PUBLISHING AG
Characterization of the structure of long RNA molecules (>1 kb) is usually a time-consuming and tedious process. In this study, we have developed an imaging procedure for obtaining images of the extended secondary structures of long RNA molecules combined with automated MATLAB-based data processing algorithms for identification of the domain architecture of the molecules in these images. These algorithms include a molecule autoselection procedure based on height and area thresholding, a morphological thinning procedure to generate skeletons of the molecule in order to analyze the branched structure of the molecules, and a procedure to generate local volume profiles along the main chain of the molecule for identification of domains and prediction of the number of nucleotides comprising each domain. The single-molecule nature of this technique also allows for the identification of varying conformations of the molecule and assessment of the conformational flexibility of the identified domain organization.
- リンク情報
- ID情報
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- DOI : 10.1007/978-3-319-46601-9_1
- ISSN : 0930-8989
- eISSN : 1867-4941
- Web of Science ID : WOS:000398624800001