MISC

2007年1月

Cathepsin E deficiency induces a novel form of lysosomal storage disorder showing the accumulation of lysosomal membrane sialoglycoproteins and the elevation of lysosomal pH in macrophages

JOURNAL OF BIOLOGICAL CHEMISTRY
  • Michiyo Yanagawa
  • ,
  • Takayuki Tsukuba
  • ,
  • Tsuyoshi Nishioku
  • ,
  • Yoshiko Okamoto
  • ,
  • Kuniaki Okamoto
  • ,
  • Ryosuke Takii
  • ,
  • Yoshihiro Terada
  • ,
  • Keiichi I. Nakayama
  • ,
  • Tomoko Kadowaki
  • ,
  • Kenji Yamamoto

282
3
開始ページ
1851
終了ページ
1862
記述言語
英語
掲載種別
DOI
10.1074/jbc.M604143200
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Cathepsin E, an endolysosomal aspartic proteinase predominantly expressed in cells of the immune system, has an important role in immune responses. However, little is known about the precise roles of cathepsin E in this system. Here we report that cathepsin E deficiency (CatE(-/-)) leads to a novel form of lysosome storage disorder in macrophages, exhibiting the accumulation of the two major lysosomal membrane sialoglycoproteins LAMP-1 and LAMP-2 and the elevation of lysosomal pH. These striking features were also found in wild-type macrophages treated with pepstatin A and Ascaris inhibitor. Whereas there were no obvious differences in their expression, biosynthesis, and trafficking between wild-type and CatE(-/-) macrophages, the degradation rates of these two membrane proteins were apparently decreased as a result of cathepsin E deficiency. Because there was no difference in the vacuolar-type H+ -ATPase activity in both cell types, the elevated lysosomal pH in CatE(-/-) macrophages is most likely due to the accumulation of these lysosomal membrane glycoproteins highly modified with acidic monosaccharides, thereby leading to the disruption of non-proton factors controlling lysosomal pH. Furthermore, the selective degradation of LAMP-1 and LAMP-2, as well as LIMP-2, was also observed by treatment of the lysosomal membrane fraction isolated from wild-type macrophages with purified cathepsin E at pH 5. Our results thus suggest that cathepsin E is important for preventing the accumulation of these lysosomal membrane sialoglycoproteins that can induce a new form of lysosomal storage disorder.

リンク情報
DOI
https://doi.org/10.1074/jbc.M604143200
CiNii Articles
http://ci.nii.ac.jp/naid/80018695661
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17095504
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000243451300036&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.M604143200
  • ISSN : 0021-9258
  • eISSN : 1083-351X
  • CiNii Articles ID : 80018695661
  • PubMed ID : 17095504
  • Web of Science ID : WOS:000243451300036

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