2019年2月
A GH family 28 endo-polygalacturonase from the brown-rot fungus Fomitopsis palustris: Purification, gene cloning, enzymatic characterization and effects of oxalate
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 123
- 号
- 開始ページ
- 108
- 終了ページ
- 116
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.ijbiomac.2018.11.004
- 出版者・発行元
- ELSEVIER
Brown-rot fungi are the wood-decay basidiomycetes and have ability to break down plant cell wall carbohydrates. It has been suggested that degradation of pectin is important for the initial stages of brown rot. We purified an endo-polygalacturonase (FpPG28A) from the brown-rot fungus Fomitopsis palustris, analysis of the predicted amino acid sequence indicated that FpPG28A belongs to GH family 28. The highest activity of purified FpPG28A was observed at 60 degrees C in 50 mM sodium acetate buffer (pH 5.0); this activity was highly specific for polygalacturonic acid chains. However, calcium polygalacturonate gel was not degraded by FpPG28A under those optimal conditions. We observed that calcium polygalacturonate gel was readily degraded by the enzyme in the oxalate buffer. Furthermore, the thermostability of FpPG28A was elevated in oxalate buffer at pH 3.0. These results indicated that oxalate has an important role in the degradation of woody pectin by FpPG28A. (C) 2018 Elsevier B.V. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.ijbiomac.2018.11.004
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/30399383
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000456760100013&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-85056459069&partnerID=MN8TOARS
- ID情報
-
- DOI : 10.1016/j.ijbiomac.2018.11.004
- ISSN : 0141-8130
- eISSN : 1879-0003
- ORCIDのPut Code : 70790309
- PubMed ID : 30399383
- SCOPUS ID : 85056459069
- Web of Science ID : WOS:000456760100013