論文

査読有り
2014年7月

In Vitro Synthesis of the E. coli Sec Translocon from DNA

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
  • Hideaki Matsubayashi
  • ,
  • Yutetsu Kuruma
  • ,
  • Takuya Ueda

53
29
開始ページ
7535
終了ページ
7538
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/anie.201403929
出版者・発行元
WILEY-V C H VERLAG GMBH

Difficulties in constructing complex lipid/protein membranes have severely limited the development of functional artificial cells endowed with vital membrane-related functions. The Sec translocon membrane channel, which mediates the insertion of membrane proteins into the plasma membrane, was constructed in the membrane of lipid vesicles through in vitro expression of its component proteins. The components of the Sec translocon were synthesized from their respective genes in the presence of liposomes, thereby bringing about a functional complex. The synthesized E. coli Sec translocon mediated the membrane translocation of single- and multi-span membrane proteins. The successful translocation of a functional peptidase into the liposome lumen further confirmed the proper insertion of the translocon complex. Our results demonstrate the feasible construction of artificial cells, the membranes of which can be functionalized by directly decoding genetic information into membrane functions.

Web of Science ® 被引用回数 : 44

リンク情報
DOI
https://doi.org/10.1002/anie.201403929
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24894900
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339564800021&DestApp=WOS_CPL

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