論文

査読有り
2014年

The PURE system for protein production

Methods in Molecular Biology
  • Yoshihiro Shimizu
  • ,
  • Yutetsu Kuruma
  • ,
  • Takashi Kanamori
  • ,
  • Takuya Ueda

1118
開始ページ
275
終了ページ
284
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-1-62703-782-2_19
出版者・発行元
Humana Press Inc.

In the field of molecular biology or biochemistry, preparation and use of purified proteins involved in a certain biological system is crucial for understanding their mechanisms and functions in cells or organisms. The recent progress in a cell-free translation system allows us to prepare proteins in a test tube directly from cDNAs that encode the amino acid sequences. The use of the reconstituted cell-free translation system termed PURE (Protein synthesis Using Recombinant Elements) for these purposes is effective in several applications. Here we describe methods of recombinant protein expression using the PURE system for molecular biological or biochemical studies. © 2014 Springer Science+Business Media, LLC.

リンク情報
DOI
https://doi.org/10.1007/978-1-62703-782-2_19
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24395424

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