論文

査読有り
2013年10月

Unbiased Tracking of the Progression of mRNA and Protein Synthesis in Bulk and in Liposome-Confined Reactions

CHEMBIOCHEM
  • Pauline van Nies
  • ,
  • Zohreh Nourian
  • ,
  • Maurits Kok
  • ,
  • Roeland van Wijk
  • ,
  • Jonne Moeskops
  • ,
  • Ilja Westerlaken
  • ,
  • Jos M. Poolman
  • ,
  • Rienk Eelkema
  • ,
  • Jan H. van Esch
  • ,
  • Yutetsu Kuruma
  • ,
  • Takuya Ueda
  • ,
  • Christophe Danelon

14
15
開始ページ
1963
終了ページ
1966
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cbic.201300449
出版者・発行元
WILEY-V C H VERLAG GMBH

The compartmentalization of a cell-free gene expression system inside a self-assembled lipid vesicle is envisioned as the simplest chassis for the construction of a minimal cell. Although crucial for its realization, quantitative understanding of the dynamics of gene expression in bulk and liposome-confined reactions is scarce. Here, we used two orthogonal fluorescence labeling tools to report the amounts of mRNA and protein produced in a reconstituted biosynthesis system, simultaneously and in real-time. The Spinach RNA aptamer and its fluorogenic probe were used for mRNA detection. Applying this dual-reporter assay to the analysis of transcript and protein production inside lipid vesicles revealed that their levels are uncorrelated, most probably a consequence of the low copy-number of some components in liposome-confined reactions. We believe that the stochastic nature of gene expression should be appreciated as a design principle for the assembly of a minimal cell.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1002/cbic.201300449
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/24027226
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000325487900014&DestApp=WOS_CPL

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