論文

査読有り
2013年

Semi-synthetic minimal cells: Biochemical, physical, and technological aspects

Synthetic Biology
  • Pasquale Stano
  • ,
  • Tereza Pereira de Souza
  • ,
  • Yutetsu Kuruma
  • ,
  • Paolo Carrara
  • ,
  • Pier Luigi Luisi

開始ページ
261
終了ページ
276
記述言語
英語
掲載種別
論文集(書籍)内論文
DOI
10.1016/B978-0-12-394430-6.00014-5
出版者・発行元
Elsevier Inc.

The construction of synthetic cells is one of the major goals of bioengineering. The most successful approach consists in the encapsulation of biochemical materials (DNA, RNA, enzymes, etc.) inside lipid vesicles (liposomes), mimicking a cell structure. In this contribution, that also aims at introducing the reader to 'chemical synthetic biology,' we describe the current state of the art of 'semi-synthetic minimal cells' (SSMCs), namely, cell-like structures containing the minimal number of biological compounds that are required to reconstruct a function of interest. We will first describe how the concept of the minimal cell was originated and its relation with the theory of autopoiesis, then we review the most advanced results focused on genetic/metabolic networks inside liposomes. Next, we emphasize that relevance of physical aspects (too often neglected) that impact on the solute entrapment process, and finally we discuss new technological trends in SSMC research that will probably allow their future use in biotechnology. © 2013 Copyright © 2013 Elsevier Inc. All rights reserved.

リンク情報
DOI
https://doi.org/10.1016/B978-0-12-394430-6.00014-5

エクスポート
BibTeX RIS