論文

査読有り
2011年

New and unexpected insights on the formation of protocells from a synthetic biology approach: The case of entrapment of biomacromolecules and protein synthesis inside vesicles

The Minimal Cell: The Biophysics of Cell Compartment and the Origin of Cell Functionality
  • Pasquale Stano
  • ,
  • Tereza Pereira De Souza
  • ,
  • Matteo Allegretti
  • ,
  • Yutetsu Kuruma
  • ,
  • Pier Luigi Luisi

開始ページ
195
終了ページ
216
記述言語
英語
掲載種別
論文集(書籍)内論文
DOI
10.1007/978-90-481-9944-0_11
出版者・発行元
Springer Netherlands

In this chapter we present our semi-synthetic approach to the construction of minimal living cells. Firstly, we shortly review the advancements carried out in the last few years, especially in the field of reactions inside liposomes. Then we discuss our recent study on the investigation of minimal cell size, carried out from the perspective of synthetic biology. In particular, we examine the question of the minimal physical size of cells by using liposomes with entrapped the complete ribosomal machinery for protein expression (green fluorescence protein), and making the assumption that this size would also correspond to a full fledged cell. We have found that liposomes with radius ca. 100 nm - which is the smallest size ever considered in the literature for protein expression - are still capable of protein expression. Finally, we show how this study has provided insightful results that actually broaden our perspective and pave the way to more extensive studies on vesicle formation and encapsulation of solutes. In fact, since classic statistical analysis gives zero or negligible probability for the simultaneous entrapment of many different molecular components in one single 100 nm radius spherical compartment at the given bulk concentration, the agreement between theoretical statistical predictions and experimental data is possible by assuming that the concentration of solutes in the liposomes becomes larger by at least of a factor 20. These aspects have a great relevance in origins of life scenarios, where the formation of functional protocells is a decisive - yet not fully understood - event. We provide a final discussion on possible mechanisms and on the experimental investigations we would like to carry out in the near future. © 2011 Springer Science+Business Media B.V.

リンク情報
DOI
https://doi.org/10.1007/978-90-481-9944-0_11

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