論文

国際誌
2022年1月

De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide.

Nature nanotechnology
  • Keisuke Shimizu
  • Batsaikhan Mijiddorj
  • Masataka Usami
  • Ikuro Mizoguchi
  • Shuhei Yoshida
  • Shiori Akayama
  • Yoshio Hamada
  • Akifumi Ohyama
  • Kenji Usui
  • Izuru Kawamura
  • Ryuji Kawano
  • 全て表示

17
1
開始ページ
67
終了ページ
75
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41565-021-01008-w

The amino-acid sequence of a protein encodes information on its three-dimensional structure and specific functionality. De novo design has emerged as a method to manipulate the primary structure for the development of artificial proteins and peptides with desired functionality. This paper describes the de novo design of a pore-forming peptide, named SV28, that has a β-hairpin structure and assembles to form a stable nanopore in a bilayer lipid membrane. This large synthetic nanopore is an entirely artificial device for practical applications. The peptide forms multidispersely sized nanopore structures ranging from 1.7 to 6.3 nm in diameter and can detect DNAs. To form a monodispersely sized nanopore, we redesigned the SV28 by introducing a glycine-kink mutation. The resulting redesigned peptide forms a monodisperse pore with a diameter of 1.7 nm leading to detection of a single polypeptide chain. Such de novo design of a β-hairpin peptide has the potential to create artificial nanopores, which can be size adjusted to a target molecule.

リンク情報
DOI
https://doi.org/10.1038/s41565-021-01008-w
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34811552
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8770118
ID情報
  • DOI : 10.1038/s41565-021-01008-w
  • PubMed ID : 34811552
  • PubMed Central 記事ID : PMC8770118

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