2018年7月24日
Preparation of Nafion/Polycation Layer-by-Layer Films for Adsorption and Release of Insulin
Polymers
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- 巻
- 10
- 号
- 8
- 開始ページ
- 812
- 終了ページ
- 812
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/polym10080812
- 出版者・発行元
- MDPI AG
Thin films were prepared using layer-by-layer (LbL) deposition of Nafion (NAF) and polycations such as poly(allylamine hydrochloride) (PAH), poly(ethyleneimine) (PEI), and poly(diallydimethylammonium chloride) (PDDA). Insulin was then adsorbed on the NAF-polycation LbL films by immersion in an insulin solution. The NAF-polycation LbL films were characterized using a quartz crystal microbalance and an atomic force microscope. The release of insulin from the LbL films was characterized using UV-visible adsorption spectroscopy and fluorescence emission spectroscopy. The greatest amount of insulin was adsorbed on the NAF-PAH LbL film. The amount of insulin adsorbed on the (NAF/PAH)5NAF LbL films by immersion in a 1 mg mL−1 insulin solution at pH 7.4 was 61.8 µg cm−2. The amount of insulin released from the LbL films was higher when immersed in insulin solutions at pH 2.0 and pH 9.0 than at pH 7.4. Therefore, NAF-polycations could be employed as insulin delivery LbL films under mild conditions and as an insulin release control system according to pH change.
- ID情報
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- DOI : 10.3390/polym10080812
- eISSN : 2073-4360