論文

国際誌
2018年2月14日

ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.

Development (Cambridge, England)
  • Marina Matsumiya
  • ,
  • Takehito Tomita
  • ,
  • Kumiko Yoshioka-Kobayashi
  • ,
  • Akihiro Isomura
  • ,
  • Ryoichiro Kageyama

145
4
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1242/dev.156836

Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 oscillation is synchronized between neighboring cells, and the posterior-anterior axis is self-organized as the central-peripheral axis. This method is applicable to chemical-library screening and will facilitate the analysis of the molecular nature of the segmentation clock.

リンク情報
DOI
https://doi.org/10.1242/dev.156836
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29437832
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5869006
ID情報
  • DOI : 10.1242/dev.156836
  • PubMed ID : 29437832
  • PubMed Central 記事ID : PMC5869006

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