論文

国際誌
2013年6月

Region-specific Etv2 ablation revealed the critical origin of hemogenic capacity from Hox6-positive caudal-lateral primitive mesoderm.

Experimental hematology
  • Hiroshi Kataoka
  • ,
  • Misato Hayashi
  • ,
  • Kumiko Kobayashi
  • ,
  • Guo Ding
  • ,
  • Yosuke Tanaka
  • ,
  • Shin-Ichi Nishikawa

41
6
開始ページ
567
終了ページ
581
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.exphem.2013.02.009

Hematopoietic cells (HPCs) develop from hemogenic endothelial cells (ECs), a specialized type of ECs undergoing hematopoietic transition. However, the mesoderm origin for hemogenic ECs or HPCs has not been clarified. To examine the origin for hemogenic mesoderm, we inactivated Etv2, a master regulator for EC/HPC commitment, in specific regions. Region-specific Etv2 ablation in early mesoderm caused local EC differentiation block, resulting in the loss of specific vascular beds without compensatory migration of residual ECs into avascular area. This feature of local EC/HPC differentiation block was correlated to the hemogenic potential of each mesoderm subset. We found that caudal-lateral mesoderm of E7.5-8.5 embryos represent the pre-committed population critical for generating hemogenic ECs. Etv2 ablation in caudal-lateral mesoderm by Hoxb6 Cre or Hoxb6CreER transgene affected vitelline plexus formation and intra-aortic hematopoietic clusters. In differentiated embryonic stem cells, this mesoderm subset marked by Hoxb6-lateral mesoderm promoter showed enriched T lymphopoietic potential among Flk-1(+) cells, which could be regarded as a characteristic for definitive HPCs. These findings indicate that critical mesoderm precursors possibly for definitive type hemogenic ECs are regionally specified in primitive mesoderm, suggesting that Hoxb6(+) caudal-lateral mesoderm represents the critical source of HPCs, which are potentially useful to enrich definitive HPCs from embryonic stem cells.

リンク情報
DOI
https://doi.org/10.1016/j.exphem.2013.02.009
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23438573
ID情報
  • DOI : 10.1016/j.exphem.2013.02.009
  • PubMed ID : 23438573

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