2018年7月
Establishment and characterization of a cell line from a feline histiocytic sarcoma.
Veterinary immunology and immunopathology
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- 巻
- 201
- 号
- 開始ページ
- 72
- 終了ページ
- 76
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.vetimm.2018.05.011
- 出版者・発行元
- Elsevier B.V.
Feline histiocytic sarcoma (HS) is an aggressive and uncommon tumor originating from dendritic cells/macrophages. Here, a feline HS cell line, FHS-1, was established from a case of feline HS and characterized. Immunohistochemically, FHS-1 cells were positive for vimentin and Iba-1, and negative for MHC class II and CD163. FHS-1 cells were positive for α-naphthyl butyrate esterase staining, which was clearly inhibited by sodium fluoride. FHS-1 cells had phagocytic and antigen uptake/processing activities. Moreover, FHS-1 cells were tested for susceptibility to feline infectious peritonitis virus (FIPV) strain 79-1146; however, this cell line was not susceptible to this viral strain. Although FHS-1 cells lost the expression of MHC class II and CD163, our findings indicate that FHS-1 is a feline HS cell line that retains functional properties of dendritic cells/macrophages in terms of phagocytic and antigen uptake/processing activities. While FHS-1 cells are not suitable for in vitro study of FIP using strain 79-1146, they may be applicable for studies aimed at developing new diagnostic and therapeutic strategies for feline HS.
- リンク情報
- ID情報
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- DOI : 10.1016/j.vetimm.2018.05.011
- ISSN : 1873-2534
- ISSN : 0165-2427
- PubMed ID : 29914686
- SCOPUS ID : 85047621341