MISC

2007年9月

Rat hepatic stellate cells acquire retinoid responsiveness after activation in vitro by post-transcriptional regulation of retinoic acid receptor alpha gene expression

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
  • Yoshihiro Mezaki
  • ,
  • Kiwamu Yoshikawa
  • ,
  • Noriko Yamaguchi
  • ,
  • Mitsutaka Miura
  • ,
  • Katsuyuki Imai
  • ,
  • Shigeaki Kato
  • ,
  • Haruki Senoo

465
2
開始ページ
370
終了ページ
379
記述言語
英語
掲載種別
DOI
10.1016/j.abb.2007.06.024
出版者・発行元
ELSEVIER SCIENCE INC

Activation of hepatic stellate cells (HSCs) is a key process in liver fibrogenesis and retinoid loss is a remarkable feature of activated HSCs. However, roles of retinoids in liver fibrogenesis are obscure. We show that mRNA levels of RAR alpha, beta and gamma were decreased during rat HSC activation in vitro. However, protein levels of RAR alpha and beta were increased during HSC activation. A retinoic acid response element-containing luciferase assay indicated that HSCs became responsive to retinoids only after activation in vitro and that this response was mediated by, at least in part, RAR alpha subtype. Immunocytochemical analysis showed that RAR alpha proteins were mainly distributed in cytosol as many spots. All-trans retinoic acid treatment strongly lowered the cytosolic RAR alpha protein levels. These results indicate that rat HSCs become retinoid responsive after activation in vitro, through post-transcriptional up-regulation of RAR alpha gene expression. (C) 2007 Elsevier Inc. All rights reserved.

Web of Science ® 被引用回数 : 18

リンク情報
DOI
https://doi.org/10.1016/j.abb.2007.06.024
CiNii Articles
http://ci.nii.ac.jp/naid/80018198978
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/17693386
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000249570100008&DestApp=WOS_CPL

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