2014年
Genetically encoded FRET indicators for live-cell imaging of histone acetylation
Methods in Molecular Biology
- ,
- 巻
- 1071
- 号
- 開始ページ
- 151
- 終了ページ
- 161
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/978-1-62703-622-1_12
- 出版者・発行元
- Humana Press Inc.
Histone acetylation is dynamically and reversibly controlled by histone acetyltransferases and deacetylases during cellular events such as cell division and differentiation. However, the dynamics of histone modifications in living cells are poorly understood because of the lack of experimental tools to monitor them in a real-time fashion. Herein, we introduce Förster/fluorescence resonance energy transfer (FRET)-based indicators to visualize acetylation of histone H4, and describe a protocol for live-cell imaging with high spatiotemporal resolution. © 2014 Springer Science+Business Media, LLC.
- リンク情報
- ID情報
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- DOI : 10.1007/978-1-62703-622-1_12
- ISSN : 1064-3745
- PubMed ID : 24052387
- SCOPUS ID : 84934439503