2004年4月
Sample enrichment by using monolithic precolumns in microcolumn liquid chromatography
JOURNAL OF CHROMATOGRAPHY A
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- 巻
- 1033
- 号
- 2
- 開始ページ
- 205
- 終了ページ
- 212
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1016/j.chroma.2004.01.045
- 出版者・発行元
- ELSEVIER SCIENCE BV
An on-line sample enrichment system was designed using monolithic precolumns in microcolumn LC. The monolithic ODS capillary columns were prepared via in situ sol-gel processes. The enrichment efficiency of the monolithic columns was tested by using phthalates as the analytes. The relative standard deviations (n = 6) for the retention time, peak area and peak height were between 0.4 and 1.2%, 0.9 and 5.5% and 0.4 and 3.9%,, respectively. The system was linear (R(2) > 0.99) within the working sample concentration and sample volume ranges. Comparing to 0.2 mul injection with a typical sample injector, the theoretical plate number of a same separation column was increased by 3-6-fold when the precolumn unit was used for sample injection. The recoveries of the analytes were between 88 and 120%, and the sample volume that could be injected into the system was increased up to 5000-fold. The limits of detection were improved by more than 2000-fold and were between 0.21 and 0.87 ng ml(-1) even with a UV absorbance detector. This system was applied to the determination of phthalates contained in laboratory distilled water and tap water samples. (C) 2004 Elsevier B.V. All rights reserved.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.chroma.2004.01.045
- CiNii Articles
- http://ci.nii.ac.jp/naid/30002788856
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/15088740
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000220398900002&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=1542298915&origin=inward
- ID情報
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- DOI : 10.1016/j.chroma.2004.01.045
- ISSN : 0021-9673
- CiNii Articles ID : 30002788856
- PubMed ID : 15088740
- Web of Science ID : WOS:000220398900002