論文

査読有り 国際誌
2021年6月24日

Dynamic Shape Transformation of a DNA Scaffold Applied for an Enzyme Nanocarrier

Frontiers in Chemistry
  • Peng Lin
  • ,
  • Huyen Dinh
  • ,
  • Eiji Nakata
  • ,
  • Takashi Morii

9
開始ページ
697857
終了ページ
697857
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3389/fchem.2021.697857
出版者・発行元
Frontiers Media {SA}

<jats:p>Structural programmability and accurate addressability of DNA nanostructures are ideal characteristics for the platform of arranging enzymes with the nanoscale precision. In this study, a three-dimensional DNA scaffold was designed to enable a dynamic shape transition from an open plate-like structure to its closed state of a hexagonal prism structure. The two domains in the open state were folded together to transform into the closed state by hybridization of complementary short DNA closing keys at both of the facing edges in over 90% yield. The shape transformation of the DNA scaffold was extensively studied by means of the fluorescence energy transfer measurement, atomic force microscope images, and agarose gel electrophoretic analyses. A dimeric enzyme xylitol dehydrogenase was assembled on the DNA scaffold in its open state in a high-loading yield. The enzyme loaded on the scaffold was subsequently transformed to its closed state by the addition of short DNA closing keys. The enzyme encapsulated in the closed state displayed comparable activity to that in the open state, ensuring that the catalytic activity of the enzyme was well maintained in the DNA nanocarrier. The nanocarrier with efficient encapsulation ability is potentially applicable for drug delivery, biosensing, biocatalytic, and diagnostic tools.</jats:p>

リンク情報
DOI
https://doi.org/10.3389/fchem.2021.697857
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34249866
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263910
ID情報
  • DOI : 10.3389/fchem.2021.697857
  • ISSN : 2296-2646
  • ORCIDのPut Code : 100931152
  • PubMed ID : 34249866
  • PubMed Central 記事ID : PMC8263910

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