2022年9月
Mechanistic Aspects for The Modulation of Enzyme Reactions on the DNA Scaffold
Molecules
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- ,
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- 巻
- 27
- 号
- 19
- 記述言語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.3390/molecules27196309
Cells have developed intelligent systems to implement the complex and efficient enzyme cascade reactions via the strategies of organelles, bacterial microcompartments and enzyme complexes. The scaffolds such as the membrane or protein in the cell are believed to assist the co-localization of enzymes and enhance the enzymatic reactions. Inspired by nature, enzymes have been located on a wide variety of carriers, among which DNA scaffolds attract great interest for their programmability and addressability. Integrating these properties with the versatile DNA–protein conjugation methods enables the spatial arrangement of enzymes on the DNA scaffold with precise control over the interenzyme distance and enzyme stoichiometry. In this review, we survey the reactions of a single type of enzyme on the DNA scaffold and discuss the proposed mechanisms for the catalytic enhancement of DNA-scaffolded enzymes. We also review the current progress of enzyme cascade reactions on the DNA scaffold and discuss the factors enhancing the enzyme cascade reaction efficiency. This review highlights the mechanistic aspects for the modulation of enzymatic reactions on the DNA scaffold.
- リンク情報
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- DOI
- https://doi.org/10.3390/molecules27196309
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/36234845
- URL
- https://www.mdpi.com/1420-3049/27/19/6309
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85139863934&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85139863934&origin=inward
- ID情報
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- DOI : 10.3390/molecules27196309
- eISSN : 1420-3049
- ORCIDのPut Code : 119866916
- PubMed ID : 36234845
- SCOPUS ID : 85139863934