論文

国際誌
2016年

Independent Evolution of Winner Traits without Whole Genome Duplication in Dekkera Yeasts.

PloS one
  • Yi-Cheng Guo
  • ,
  • Lin Zhang
  • ,
  • Shao-Xing Dai
  • ,
  • Wen-Xing Li
  • ,
  • Jun-Juan Zheng
  • ,
  • Gong-Hua Li
  • ,
  • Jing-Fei Huang

11
5
開始ページ
e0155140
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0155140

Dekkera yeasts have often been considered as alternative sources of ethanol production that could compete with S. cerevisiae. The two lineages of yeasts independently evolved traits that include high glucose and ethanol tolerance, aerobic fermentation, and a rapid ethanol fermentation rate. The Saccharomyces yeasts attained these traits mainly through whole genome duplication approximately 100 million years ago (Mya). However, the Dekkera yeasts, which were separated from S. cerevisiae approximately 200 Mya, did not undergo whole genome duplication (WGD) but still occupy a niche similar to S. cerevisiae. Upon analysis of two Dekkera yeasts and five closely related non-WGD yeasts, we found that a massive loss of cis-regulatory elements occurred in an ancestor of the Dekkera yeasts, which led to improved mitochondrial functions similar to the S. cerevisiae yeasts. The evolutionary analysis indicated that genes involved in the transcription and translation process exhibited faster evolution in the Dekkera yeasts. We detected 90 positively selected genes, suggesting that the Dekkera yeasts evolved an efficient translation system to facilitate adaptive evolution. Moreover, we identified that 12 vacuolar H+-ATPase (V-ATPase) function genes that were under positive selection, which assists in developing tolerance to high alcohol and high sugar stress. We also revealed that the enzyme PGK1 is responsible for the increased rate of glycolysis in the Dekkera yeasts. These results provide important insights to understand the independent adaptive evolution of the Dekkera yeasts and provide tools for genetic modification promoting industrial usage.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0155140
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27152421
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4859550
ID情報
  • DOI : 10.1371/journal.pone.0155140
  • PubMed ID : 27152421
  • PubMed Central 記事ID : PMC4859550

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