2017年4月
Characterization and functional expression of a rubber degradation gene of a Nocardia degrader from a rubber-processing factory
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 123
- 号
- 4
- 開始ページ
- 412
- 終了ページ
- 418
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.jbiosc.2016.11.012
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
A rubber-degrading bacterial consortium named H2DA was obtained from an enrichment culture with natural rubber latex and rubber-processing factory waste in Vietnam. Gel permeation chromatography analysis revealed that only the strain NVL3 degraded synthetic poly(cis-1,4-isoprene) into low-molecular-weight intermediates among the three strains found in the H2DA. The 16S-rRNA gene sequence of NVL3 showed the highest identity with that of Nocardia farcinica DSM 43665(T). NVL3 accumulated aldehyde intermediates from synthetic poly(cis-1,4-isoprene) on a rubber-overlay plate as indicated by Schiff's staining. NVL3 also degraded deproteinized natural rubber into low-molecular-weight aldehyde intermediates. A latex-clearing protein (Icp) gene ortholog was identified within the genome sequence of NVL3, and it showed a moderate amino-acid identity (54-75%) with the Icp genes from previously reported rubber degraders. The heterologous expression of the NVL3 Icp in Escherichia coli BL21(DE3) allowed us to purify the 46.8-kDa His-tagged Icp gene product (His-Lcp). His-Lcp degraded synthetic poly(cis-1,4-isoprene) and accumulated aldehyde intermediates from deproteinized natural rubber suggesting the functional expression of the Icp gene from a Nocardia degrader in E. coli. Quantitative reverse transcription PCR analysis indicated the strong transcriptional induction of the lcp gene in NVL3 in the presence of synthetic poly(cis-1,4-isoprene). These results suggest the involvement of the lcp gene in rubber degradation in NVL3.
- リンク情報
- ID情報
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- DOI : 10.1016/j.jbiosc.2016.11.012
- ISSN : 1389-1723
- eISSN : 1347-4421
- Web of Science ID : WOS:000401048800002