2013年
Genetically encoded system to track histone modification in vivo
Scientific Reports
- 巻
- 3
- 号
- 開始ページ
- 2436
- 終了ページ
- 2436
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1038/srep02436
- 出版者・発行元
- NATURE PUBLISHING GROUP
Post-translational histone modifications play key roles in gene regulation, development, and differentiation, but their dynamics in living organisms remain almost completely unknown. To address this problem, we developed a genetically encoded system for tracking histone modifications by generating fluorescent modification-specific intracellular antibodies (mintbodies) that can be expressed in vivo. To demonstrate, an H3 lysine 9 acetylation specific mintbody (H3K9ac-mintbody) was engineered and stably expressed in human cells. In good agreement with the localization of its target acetylation, H3K9ac-mintbody was enriched in euchromatin, and its kinetics measurably changed upon treatment with a histone deacetylase inhibitor. We also generated transgenic fruit fly and zebrafish stably expressing H3K9ac-mintbody for in vivo tracking. Dramatic changes in H3K9ac-mintbody localization during Drosophila embryogenesis could highlight enhanced acetylation at the start of zygotic transcription around mitotic cycle 7. Together, this work demonstrates the broad potential of mintbody and lays the foundation for epigenetic analysis in vivo.
- リンク情報
-
- DOI
- https://doi.org/10.1038/srep02436
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/23942372
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3743053
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000323097900004&DestApp=WOS_CPL
- Scopus
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84882761820&origin=inward 本文へのリンクあり
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84882761820&origin=inward
- ID情報
-
- DOI : 10.1038/srep02436
- ISSN : 2045-2322
- eISSN : 2045-2322
- PubMed ID : 23942372
- PubMed Central 記事ID : PMC3743053
- SCOPUS ID : 84882761820
- Web of Science ID : WOS:000323097900004