Takahashi Masayuki - My portal

Profile Information

Affiliation: Assistant Professor, Niigata University of Pharmacy and Applied Life Sciences

Degree: 博士(応用生命科学)(Mar, 2014, 新潟薬科大学大学院)
修士(応用生命科学)(Mar, 2011, 新潟薬科大学大学院)
学士(応用生命科学)(Mar, 2009, 新潟薬科大学)

Researcher number: 30743778
J-GLOBAL ID: 201401084829806956
researchmap Member ID: B000239073

Research Interests

Research Areas

Research History

Jul, 2018 - Present

Niigata University of Pharmacy and Applied Life Sciences
Jul, 2018 - Present

Research Institute for Healthy Living, Niigata University of Pharmacy and Applied Life Sciences
Apr, 2018 - Jun, 2018

Special researcher of the Japan Society for the Promotion of Science
Apr, 2018 - Jun, 2018

Special researcher of the Japan Society for the Promotion of Science
Oct, 2016 - Mar, 2018

Researcher
Oct, 2016 - Mar, 2018

Researcher
Sep, 2015 - Sep, 2016

Assistant Professor
Sep, 2015 - Sep, 2016

Assistant Professor
Jun, 2014 - Aug, 2015

Researcher
Jun, 2014 - Aug, 2015

Researcher
Apr, 2014 - May, 2014

Researcher
Apr, 2011 - Mar, 2014

Special researcher of the Japan Society for the Promotion of Science

Education

Apr, 2011 - Mar, 2014

Niigata University of Pharmacy and Applied Life Sciences
Apr, 2009 - Mar, 2011

Niigata University of Pharmacy and Applied Life Sciences
Apr, 2006 - Mar, 2009

Niigata University of Pharmacy and Applied Life Sciences
Apr, 2003 - Mar, 2006

普通科, 新潟県立三条高等学校

Awards

Papers

Genes for tRNA recycling are upregulated in response to infection with Theiler's mouse encephalitis virus

Mineaki Seki, Akihiko Komuro, Tatsuya Ishikawa, Masayuki Takahashi, Masayuki Nashimoto

Biochemical and Biophysical Research Communications, Jan, 2022 Peer-reviewed
Transcription from the proximal promoter of ELAC1, a gene for tRNA repair, is upregulated by interferons

Mineaki Seki, Akihiko Komuro, Masayuki Takahashi, Masayuki Nashimoto

Biochemical and Biophysical Research Communications, 583(31) 162-168, Dec, 2021 Peer-reviewed
Perturbing the Normal Level of SIDT1 Suppresses the Naked ASO Effect

Masayuki Takahashi, Mineaki Seki, Masayuki Nashimoto, Tomohiro Kabuta

Journal of Nucleic Acids, 2021(Article ID 2458470 https://doi.org/10.1155/2021/2458470, Nov, 2021 Peer-reviewedLead authorCorresponding author
A naked antisense oligonucleotide with phosphorothioate linkages is taken up intracellularly more efficiently but functions less effectively

Masayuki Takahashi, Mineaki Seki, Masayuki Nashimoto

Biochem Biophys Res Commun, 573(8) 140-144, Oct, 2021 Peer-reviewedLead author
The heptamer sgRNA targeting the human OCT4 mRNA can upregulate the OCT4 expression

Tadasuke Nozaki*, Masayuki Takahashi*, Tatsuya Ishikawa*, Arisa Haino, Mineaki Seki, Hidetomo Kikuchi, Bo Yuan, Masayuki Nashimoto (* first equally)

Biochemistry and Biophysics Reports, in press, Jan, 2021 Peer-reviewedLead author
RNA-hydrolyzing activity of metallo-β-lactamase IMP-1

Yoshiki Kato, Masayuki Takahashi, Mineaki Seki, Masayuki Nashimoto, Akiko Shimizu-Ibuka

PLoS One., 15(10) e0241557, Oct, 2020 Peer-reviewed
Heptamer-type small guide RNA that can shift macrophages toward the M1 state.

Ishikawa T, Haino A, Ichiyanagi T, Takahashi M, Seki M, Nashimoto M.

Blood Cells Mol Dis., doi: 10.1016/j.bcmd.2020.102503(Epub ahead of print.) PMID: 32920464., Sep, 2020 Peer-reviewed
Cytosolic domain of SIDT2 carries an arginine-rich motif that binds to RNA/DNA and is important for the direct transport of nucleic acids into lysosomes.

Katsunori Hase, Viorica Raluca Contu, Chihana Kabuta, Ryohei Sakai, Masayuki Takahashi, Naoyuki Kataoka, Fumihiko Hakuno, Shin-Ichiro Takahashi, Yuuki Fujiwara, Keiji Wada, Tomohiro Kabuta

Autophagy, in press(11) 1-15, Jan 16, 2020 Peer-reviewed
Potential physiological roles of the 31/32-nucleotide Y4-RNA fragment in human plasma

Sho Ninomiya, Tatsuya Ishikawa, Masayuki Takahashi, MineakiSeki, MasayukiNashimoto

Non-coding RNA Research, in press, Nov, 2019 Peer-reviewed
Dual activity of PNGM-1 pinpoints the evolutionary origin of subclass B3 metallo-β-lactamases: a molecular and evolutionary study

Jung Hun Lee, Masayuki Takahashi, Jeong Ho Jeon, Lin-Woo Kang, Mineaki Seki, Kwang Seung Park, Myoung-Ki Hong, Yoon Sik Park, Tae Yeong Kim, Asad Mustafa Karim, Jung-Hyun Lee, Masayuki Nashimoto, Sang Hee Lee, first equally

Emerging Microbes & Infections, 8(1) 1688-1700, Nov, 2019 Peer-reviewedLead author
Lysosomal targeting of SIDT2 via multiple YXXΦ motifs is required for SIDT2 function in the process of RNautophagy

Contu VR, Hase K, Kozuka-Hata H, Oyama M, Fujiwara Y, Kabuta C, Takahashi M, Hakuno F, Takahashi SI, Wada K, Kabuta T

Journal of cell science, 130 2843-2853, Jul, 2017 Peer-reviewed
SIDT2 mediates gymnosis, the uptake of naked single-stranded oligonucleotides into living cells.

Takahashi M, Contu VR, Kabuta C, Hase K, Fujiwara Y, Wada K, Kabuta T.

RNA Biology, 14 1534-1543, Mar, 2017 Peer-reviewedLead author
Lysosomal putative RNA transporter SIDT2 mediates direct uptake of RNA by lysosomes

Shu Aizawa, Yuuki Fujiwara, Viorica Raluca Contu, Katsunori Hase, Masayuki Takahashi, Hisae Kikuchi, Chihana Kabuta, Keiji Wada, Tomohiro Kabuta

AUTOPHAGY, 12(3) 565-578, 2016 Peer-reviewed
Potential Small Guide RNAs for tRNase Z(L) from Human Plasma, Peripheral Blood Mononuclear Cells, and Cultured Cell Lines

Sho Ninomiya, Mitsuoki Kawano, Takashi Abe, Tatsuya Ishikawa, Masayuki Takahashi, Masato Tamura, Yoshiaki Takahashi, Masayuki Nashimoto

PLOS ONE, 10(3) e0118631., Mar, 2015 Peer-reviewed
Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

Masayuki Takahashi, Reyad A. Elbarbary, Norihiro Watanabe, Atsushi Goto, Daichi Kamiya, Yoshihiro Watabe, Takayoshi Uchiyama, Miwako Narita, Masuhiro Takahashi, Yoshiaki Takahashi, Noriko Ishihara, Tatsuya Miyazawa, Tetsuo Yoshida, Mitsuoki Kawano, Masato Tamura, Masayuki Nashimoto

LEUKEMIA RESEARCH, 38(7) 808-815, Jul, 2014 Lead author
A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Masayuki Takahashi, Reyad A. Elbarbary, Aiko Nakashima, Mayumi Abe, Norihiro Watanabe, Miwako Narita, Masuhiro Takahashi, Masato Tamura, Tetsuo Yoshida, Masayuki Nashimoto

Cancer Letters, 328(2) 362-368, Jan 28, 2013 Peer-reviewedLead author
Elimination of Specific miRNAs by Naked 14-nt sgRNAs

Masayuki Takahashi, Reyad A. Elbarbary, Mayumi Abe, Mari Sato, Tetsuo Yoshida, Yoji Yamada, Masato Tamura, Masayuki Nashimoto

PLOS ONE, 7(6) e38496, Jun, 2012 Peer-reviewedLead author
Expanding the utility of heptamer-type sgRNA for TRUE gene silencing

Takako Sano, Masayuki Takahashi, Tadasuke Nozaki, Yoshiaki Takahashi, Masato Tamura, Masayuki Nashimoto

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 416(3-4) 427-432, Dec, 2011 Peer-reviewed
Modulation of Gene Expression by Human Cytosolic tRNase Z(L) through 5 '-Half-tRNA

Reyad A. Elbarbary, Hiroaki Takaku, Naoto Uchiumi, Hiroko Tamiya, Mayumi Abe, Masayuki Takahashi, Hiroshi Nishida, Masayuki Nashimoto

PLOS ONE, 4(6) e5908, Jun, 2009 Peer-reviewed
Regulation of the human tRNase ZS gene expression

Masayuki Takahashi, Hiroaki Takaku, Masayuki Nashimoto.

FEBS letters, 582 2532-2536, Jun, 2008 Peer-reviewedLead author

Presentations

Mechanism of the uptake of naked single-stranded oligonucleotides into living cells

Masayuki Takhashi

The 34 th Annual Meeting of the Japanese Society for the Study of Xenobiotics in Tsukuba, Dec, 2019 Invited
Gymnosis関連分子とメカニズムの解明

高橋昌幸, Viorica Raluca Contu, 株田千華, 長谷勝徳, 藤原悠紀, 株田智弘

第22回バイオ治療法研究会学術集会, Dec, 2018
裸の1本鎖オリゴの細胞内への取り込みメカニズム

高橋昌幸, Viorica Raluca Contu, 和田圭司, 株田智弘

第 17 回日本RNA学会年会, Jul, 2015
ヒトの血漿、末梢血単核細胞、培養細胞株由来のtRNase ZLをガイドする小さなRNA

石川達矢, 二宮翔, 川野光興, 阿部貴志, 高橋昌幸, 田村正人, 高橋由明, 梨本正之

第56回新潟生化学懇話会, Jun, 2015
ヒト Bcl-2 mRNA を標的とする裸の RNA heptamer によるアポトーシス誘導

高橋昌幸, 阿部真由美, 中島愛子, 野崎忠輔, リアド・エルバリバリ, 渡辺紀宏, 西田浩志, 佐野峻子, 高久洋暁, 成田美和子, 高橋益廣, 田村正人, 梨本正之

第 11 回日本 RNA 学会年会

Professional Memberships

2009

RNA学会

Research Projects

Gymnosis関連分子とメカニズムの解明

2018年度武田科学振興財団医学系研究助成(基礎), Jul, 2018 - Present

高橋昌幸
新規gymnosis関連分子Ly6aの解析とメカニズムの解明

日本学術振興会, Apr, 2020
Gymnosisの関連分子とメカニズムの解明

平成30年度特別研究員奨励費(特別研究員PD), Apr, 2018 - Jun, 2018
1本鎖核酸の細胞内への取り込みメカニズムの解明

若手研究(B), Apr, 2015 - Mar, 2017
多発性骨髄腫治療用sgRNA薬の開発

平成23年度特別研究員奨励費(特別研究員DC-1), Apr, 2011 - Mar, 2014