論文

査読有り
2015年3月

Potential Small Guide RNAs for tRNase Z(L) from Human Plasma, Peripheral Blood Mononuclear Cells, and Cultured Cell Lines

PLOS ONE
  • Sho Ninomiya
  • ,
  • Mitsuoki Kawano
  • ,
  • Takashi Abe
  • ,
  • Tatsuya Ishikawa
  • ,
  • Masayuki Takahashi
  • ,
  • Masato Tamura
  • ,
  • Yoshiaki Takahashi
  • ,
  • Masayuki Nashimoto

10
3
開始ページ
e0118631.
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0118631
出版者・発行元
PUBLIC LIBRARY SCIENCE

Several pieces of evidence suggest that small RNA degradation products together with tRNase Z(L) appear to form another layer of the whole gene regulatory network. The degraded RNAsuch as a 5'-half-tRNA and an rRNA fragment function as small guide RNA (sgRNA) to guide the enzyme to target RNA. We were curious whether there exist RNAs in plasma that can function as sgRNAs for tRNase Z(L), whether these RNAs are working as signaling molecules between cells to fulfill physiological roles, and whether there are any differences in plasma sgRNA species and levels between normal and pathological conditions. Here, we analyzed small plasma RNAs from three healthy persons and three multiple myeloma patients for potential sgRNAs by deep sequencing. We also examined small RNAs from peripheral blood mononuclear cells (PBMC) of three healthy persons and three myeloma patients and from various cultured human cell lines for sgRNAs. We found that read-number distribution patterns of plasma and PBMC RNAs differ between persons in the range of 5-40 nt and that there are many RNA species that exist significantly more or less abundantly in the plasma or PBMC of the myeloma patients than those of the healthy persons. Furthermore, we found that there are many potential sgRNAs in the 5-40-nt RNAs and that, among them, a 31-nt RNA fragment derived from 94-nt Y4-RNA, which can function as a 50-half-tRNA-type sgRNA, is overwhelmingly abundant in the plasma of 2/3 of the examinees. These observations suggest that the gene regulatory network via tRNase Z(L) and sgRNA may be extended intercellularly.


リンク情報
DOI
https://doi.org/10.1371/journal.pone.0118631
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000350684900022&DestApp=WOS_CPL
URL
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0118631
ID情報
  • DOI : 10.1371/journal.pone.0118631
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000350684900022

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