論文

筆頭著者
2014年7月

Screening of a heptamer-type sgRNA library for potential therapeutic agents against hematological malignancies

LEUKEMIA RESEARCH
  • Masayuki Takahashi
  • ,
  • Reyad A. Elbarbary
  • ,
  • Norihiro Watanabe
  • ,
  • Atsushi Goto
  • ,
  • Daichi Kamiya
  • ,
  • Yoshihiro Watabe
  • ,
  • Takayoshi Uchiyama
  • ,
  • Miwako Narita
  • ,
  • Masuhiro Takahashi
  • ,
  • Yoshiaki Takahashi
  • ,
  • Noriko Ishihara
  • ,
  • Tatsuya Miyazawa
  • ,
  • Tetsuo Yoshida
  • ,
  • Mitsuoki Kawano
  • ,
  • Masato Tamura
  • ,
  • Masayuki Nashimoto

38
7
開始ページ
808
終了ページ
815
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.leukres.2014.03.021
出版者・発行元
PERGAMON-ELSEVIER SCIENCE LTD

tRNase-Z(L)-utilizing efficacious (TRUE) gene silencing is an RNA-mediated gene expression control technology that has therapeutic potential. This technology is based on the property of tRNase Z(L) that it can cleave any target RNA at any desired site under the direction of an appropriate artificial small guide RNA (sgRNA). To search for novel potential therapeutic sgRNAs for hematological malignancies, we screened a library composed of 156 sgRNAs, and found that 20 sgRNAs can efficiently induce apoptosis in leukemia and/or myeloma cells. Furthermore, we demonstrated that 4 of the 20 sgRNAs can reduce growth rates of HL60 cells in mouse xenograft models. (C) 2014 Elsevier Ltd. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.leukres.2014.03.021
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000339339400014&DestApp=WOS_CPL
URL
https://www.sciencedirect.com/science/article/pii/S0145212614000964?via%3Dihub
ID情報
  • DOI : 10.1016/j.leukres.2014.03.021
  • ISSN : 0145-2126
  • Web of Science ID : WOS:000339339400014

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