論文

査読有り 筆頭著者
2012年6月

Elimination of Specific miRNAs by Naked 14-nt sgRNAs

PLOS ONE
  • Masayuki Takahashi
  • ,
  • Reyad A. Elbarbary
  • ,
  • Mayumi Abe
  • ,
  • Mari Sato
  • ,
  • Tetsuo Yoshida
  • ,
  • Yoji Yamada
  • ,
  • Masato Tamura
  • ,
  • Masayuki Nashimoto

7
6
開始ページ
e38496
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0038496
出版者・発行元
PUBLIC LIBRARY SCIENCE

tRNase Z(L)-utilizing efficacious gene silencing (TRUE gene silencing) is a newly developed technology to suppress mammalian gene expression. TRUE gene silencing works on the basis of a unique enzymatic property of mammalian tRNase Z(L), which is that it can recognize a pre-tRNA-like or micro-pre-tRNA-like complex formed between target RNA and artificial small guide RNA (sgRNA) and can cleave any target RNA at any desired site. There are four types of sgRNA, 5'-half-tRNA, RNA heptamer, hook RNA, and similar to 14-nt linear RNA. Here we show that a 14-nt linear-type sgRNA against human miR-16 can guide tRNase Z(L) cleavage of miR-16 in vitro and can downregulate the miR-16 level in HEK293 cells. We also demonstrate that the 14-nt sgRNA can be efficiently taken up without any transfection reagents by living cells and can exist stably in there for at least 24 hours. The naked 14-nt sgRNA significantly reduced the miR-16 level in HEK293 and HL60 cells. Three other naked 14-nt sgRNAs against miR-142-3p, miR-206, and miR-19a/b are also shown to downregulate the respective miRNA levels in various mammalian cell lines. Our observations suggest that in general we can eliminate a specific cellular miRNA at least by similar to 50% by using a naked 14-nt sgRNA on the basis of TRUE gene silencing.


リンク情報
DOI
https://doi.org/10.1371/journal.pone.0038496
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000305341700081&DestApp=WOS_CPL
URL
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038496
ID情報
  • DOI : 10.1371/journal.pone.0038496
  • ISSN : 1932-6203
  • Web of Science ID : WOS:000305341700081

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