論文

査読有り
2011年12月

Expanding the utility of heptamer-type sgRNA for TRUE gene silencing

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
  • Takako Sano
  • ,
  • Masayuki Takahashi
  • ,
  • Tadasuke Nozaki
  • ,
  • Yoshiaki Takahashi
  • ,
  • Masato Tamura
  • ,
  • Masayuki Nashimoto

416
3-4
開始ページ
427
終了ページ
432
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.bbrc.2011.11.091
出版者・発行元
ACADEMIC PRESS INC ELSEVIER SCIENCE

tRNase Z(L)-utilizing efficacious gene silencing (TRUE gene silencing) is a novel technology for suppressing gene expression. TRUE gene silencing is based on a unique enzymatic property of mammalian tRNase Z(L), which is that it can cleave any target RNA at any desired site by recognizing a pre-tRNA-like or micro-pre-tRNA-like complex formed between the target RNA and artificial small guide RNA (sgRNA). sgRNA is divided into four groups, 5'-half-tRNA, RNA heptamer, hook RNA, and similar to 14-nt linear RNA. One of the final destinations of TRUE gene silencing is to generate cancer therapeutic sgRNAs, and from a pharmacological point of view, heptamer-type sgRNA appears to be the most appropriate for this purpose. In this paper, we present two strategies to expand the utility of heptamer-type sgRNA: one is about locked nucleic acid (LNA) modifications of heptamers and the other is about usage of double heptamers. We showed that RNA heptamers with LNA modifications can work as sgRNA in vitro and in vivo. We also demonstrated that two consecutively aligned heptamers can guide target RNA cleavage by human tRNase Z(L) as efficiently as a corresponding 14-nt sgRNA in vitro and that a double heptamer can work much better than a 14-nt sgRNA in vivo. (C) 2011 Elsevier Inc. All rights reserved.


リンク情報
DOI
https://doi.org/10.1016/j.bbrc.2011.11.091
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000298827800034&DestApp=WOS_CPL
URL
https://www.sciencedirect.com/science/article/pii/S0006291X11021127?via%3Dihub
ID情報
  • DOI : 10.1016/j.bbrc.2011.11.091
  • ISSN : 0006-291X
  • Web of Science ID : WOS:000298827800034

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