2015年2月
Genetic and serological characterization of chrysanthemum stem necrosis virus, a member of the genus Tospovirus
ARCHIVES OF VIROLOGY
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- 巻
- 160
- 号
- 2
- 開始ページ
- 529
- 終了ページ
- 536
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1007/s00705-014-2287-9
- 出版者・発行元
- SPRINGER WIEN
Chrysanthemum stem necrosis virus (CSNV) is a member of a tentative tospovirus species. In this study, the complete genomic sequence of the Japanese CSNV isolate TcCh07A was determined. The L RNA is 8960 nt long and encodes the 331.0-kDa RNA-dependent RNA polymerase. The M RNA is 4828 nt long and encodes the 34.1-kDa movement protein (NSm) and the 127.7-kDa glycoprotein precursor (Gn/Gc). The S RNA is 2949 nt long and encodes the 52.4-kDa silencing suppressor protein (NSs) and the 29.3-kDa nucleocapsid (N) protein. The N protein of CSNV-TcCh07A was purified from virus-infected plant tissues and used for production of a rabbit polyclonal antiserum (RAs) and a monoclonal antibody (MAb). Results of serological tests by indirect ELISA and western blotting using the prepared RAs and MAb and a previously produced RAs against the N protein of tomato spotted wilt virus (TSWV) indicated that CSNV-TcCh07A, TSWV, tomato chlorotic spot virus, groundnut ringspot virus, alstroemeria necrotic streak virus and impatiens necrotic spot virus are serologically related.
- リンク情報
- ID情報
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- DOI : 10.1007/s00705-014-2287-9
- ISSN : 0304-8608
- eISSN : 1432-8798
- Web of Science ID : WOS:000349433500022