In this study, quartz crystal microbalance (QCM) sensor based on 2, 4, 6-trinitrotoluene (TNT) recognition peptide functionalized single-walled carbon nanotubes (SWCNTs) for TNT explosive detection was developed. The SWCNTs were immobilized on the self-assembled monolayer (SAM) cysteamine modified QCM gold-chip surface. Through p-stacking interaction, the specific TNT recognition peptide (named TNTHCDR3) was anchored on the SWCNTs surface. The results revealed that the peptide-SWCNTs hybrid modified QCM sensor offered highly selective detection of TNT explosive.