2020年11月6日
Aquipluma nitroreducens gen. nov. sp. nov., a novel facultatively anaerobic bacterium isolated from a freshwater lake
International Journal of Systematic and Evolutionary Microbiology
- ,
- ,
- 巻
- 70
- 号
- 12
- 開始ページ
- 6408
- 終了ページ
- 6413
- 記述言語
- 掲載種別
- 研究論文（学術雑誌）
- DOI
- 10.1099/ijsem.0.004551
- 出版者・発行元
- Microbiology Society
A novel facultatively anaerobic, nitrate-reducing bacterium, designated MeG22T, was isolated from a freshwater lake in Japan. Cells of the strain were straight rods (0.8×2.5–10 µm), motile, and Gram-stain-negative. For growth, the optimum NaCl concentration was 0 % and the optimum temperature was 30 °C. Under anoxic conditions, strain MeG22T reduced nitrate to nitrite. Major cellular fatty acids were C15 : 1 ω6<italic>c</italic> (13.6 %), C17 : 0 (11.9 %), anteiso-C15 : 0 (10.6 %) and iso-C15 : 0 (10.6 %). The major respiratory quinone was menaquinone-7. The genome sequence of strain MeG22T consists of 5 712 279 bp with a G+C content of 40.3 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that the novel strain belonged to the family <italic>
<named-content content-type="family">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.25238" xlink:type="simple">Prolixibacteraceae</ext-link>
</named-content>
</italic> within the phylum <italic>
<named-content content-type="phylum">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.7927" xlink:type="simple">Bacteroidetes</ext-link>
</named-content>
</italic>. The closest relative of strain MeG22T was <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24105" xlink:type="simple">Sunxiuqinia faeciviva</ext-link>
</named-content>
</italic> strain JAM-BA0302T with a 16S rRNA gene sequence similarity of 90.9 %. On the basis of phylogenetic and phenotypic characterization, <italic>Aquipluma nitroreducens</italic>, gen. nov., sp. nov., belonging to the family <italic>
<named-content content-type="family">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.25238" xlink:type="simple">Prolixibacteraceae</ext-link>
</named-content>
</italic> is proposed with the type strain MeG22T (=NBRC 112896T=DSM 106262T).
<named-content content-type="family">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.25238" xlink:type="simple">Prolixibacteraceae</ext-link>
</named-content>
</italic> within the phylum <italic>
<named-content content-type="phylum">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.7927" xlink:type="simple">Bacteroidetes</ext-link>
</named-content>
</italic>. The closest relative of strain MeG22T was <italic>
<named-content content-type="species">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.24105" xlink:type="simple">Sunxiuqinia faeciviva</ext-link>
</named-content>
</italic> strain JAM-BA0302T with a 16S rRNA gene sequence similarity of 90.9 %. On the basis of phylogenetic and phenotypic characterization, <italic>Aquipluma nitroreducens</italic>, gen. nov., sp. nov., belonging to the family <italic>
<named-content content-type="family">
<ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.25238" xlink:type="simple">Prolixibacteraceae</ext-link>
</named-content>
</italic> is proposed with the type strain MeG22T (=NBRC 112896T=DSM 106262T).
- リンク情報
- ID情報
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- DOI : 10.1099/ijsem.0.004551
- ISSN : 1466-5026
- eISSN : 1466-5034